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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Antioxidant activity of fractionated foxtail millet protein hydrolysate
International Food Research Journal, Volume 19, No. 1, Year 2012
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Description
The objective of the study was to assess the antioxidant potential of defatted foxtail millet protein hydrolysates (DFMPH). Gel filtration (Sephadex G-25) chromatography was used to fractionate DFMPH. The amino acid composition, ABTS, DPPH, inhibition of linoleic acid autoxidation, metal-chelating free radical scavenging ability, and reducing power were tested to determine their antioxidant potency. The antioxidant activity of fraction (FIV) (85.71%) was closer to that of α-tocopherol (86.27%) but lower than that of BHT (butylated hydroxytoluene) (92.44%) in the linoleic acid oxidation system. Furthermore, FIV exhibited higher ABTS (2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH (1,1-diphenyl-2-picrylhydrazyl) and metal-chelating activity than the other fractions with a significant difference (P < 0.05). Amino acid profile revealed that FIV, with the strongest antioxidant activity, had the highest hydrophobic amino acids content (51.94%) and hydrophobicity (8.62 kJ/moL amino acid residue "AAR"). Molecular weight of the fractions varied from 77-1042 Da. The data obtained by the in vitro systems established antioxidant potency of DFMPH fractions. © 2008 IFRJ, Faculty of Food Science & Technology, UPM.
Authors & Co-Authors
Mohamed, Tabita Kamara
China, Wuxi
Jiangnan University
Sierra Leone, Freetown
Njala University
Issoufou, Amadou
China, Wuxi
Jiangnan University
Zhou, Huiming
China, Wuxi
Jiangnan University
Statistics
Citations: 3
Authors: 3
Affiliations: 2
Identifiers
ISSN:
19854668
Research Areas
Food Security