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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Whole blood interferon-gamma responses to Mycobacterium tuberculosis antigens in young household contacts of persons with tuberculosis in Uganda
PLoS ONE, Volume 3, No. 10, Article e3407, Year 2008
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Description
Background: Due to immunologic immaturity, IFN-γ-producing T cell responses may be decreased in young children compared to adults, thus we hypothesized that IFN-γ responses to mycobacterial antigens in household contacts exposed to Mycobacterium tuberculosis (Mtb) would be impaired in young children relative to adults. The objective of this study was to compare whole blood IFN-γ production in response to mycobacterial antigens between children and adults in Uganda. Methodology/Principal Findings: We studied household contacts of persons with culture-positive pulmonary tuberculosis (TB) enrolled in a cohort study conducted in Kampala, Uganda. Whole blood IFN-γ production in response to Mtb culture-filtrate antigens was measured by ELISA and compared between infants (<2 years old, n=80), young children (2 <5 years old, n=216), older children (5 <15 years old, n= 443) and adults (≥15 years old, n=528). We evaluated the relationship between IFN-γ responses and the tuberculin skin test (TST), and between IFN-γ responses and epidemiologic factors that reflect exposure to Mtb, and the effect of prior BCG vaccination on IFN-γ responses. Young household contacts demonstrated robust IFN-γ responses comparable to those of adults that were associated with TST and known risk factors for infection. There was no effect of prior BCG immunization on the IFN-γ response. Conclusions/Significance: Young children in a TB endemic setting can mount robust IFN-γ responses generally comparable to those of adults, and as in adults, these responses correlated with the TST and known epidemiologic risk factors for Mtb infection. © 2008 Lewinsohn et al.
Authors & Co-Authors
Lewinsohn, Deborah Anne
United States, Portland
Doernbecher Children's Hospital
Zalwango, Sarah K.
Uganda, Kampala
Uganda-cwru Research Collaboration
Stein, Catherine M.
United States, Cleveland
Case School of Medicine
Uganda, Kampala
School of Medicine, Makerere University College of Health Sciences
Mayanja-Kizza, Harriet
Uganda, Kampala
Uganda-cwru Research Collaboration
Uganda, Kampala
School of Medicine, Makerere University College of Health Sciences
Okwera, Alphonse
Uganda, Kampala
Uganda-cwru Research Collaboration
Uganda, Kampala
Mulago Hospital
Boom, W. Henry
United States, Cleveland
Case School of Medicine
Mugerwa, Roy D.
Uganda, Kampala
Uganda-cwru Research Collaboration
Uganda, Kampala
School of Medicine, Makerere University College of Health Sciences
Whalen, Christopher Curtis
United States, Cleveland
Case School of Medicine
United States, Athens
University of Georgia
Statistics
Citations: 43
Authors: 8
Affiliations: 6
Identifiers
Doi:
10.1371/journal.pone.0003407
e-ISSN:
19326203
Research Areas
Infectious Diseases
Maternal And Child Health
Study Design
Cohort Study
Study Approach
Quantitative
Study Locations
Uganda