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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Nucleotide sequence analysis of mRNAs predicts that rat pituitary and gonadal gonadotropin-releasing hormone receptor proteins have identical primary structure
Biochemical and Biophysical Research Communications, Volume 200, No. 3, Year 1994
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Description
In order to examine whether the paradoxical, extrapituitary ovarian and testicular gonadotropin-releasing hormone binding activity in rat was related to conventional pituitary GnRH receptor, the presence and nature of GnRH receptor gene transcripts were analysed by a reverse transcription-coupled polymerase chain reaction method. Different primer pairs designed to amplify regions of mRNA encompassing the complete open reading frame plus a large part of the 3'-untranslated region revealed the presence of GnRH receptor transcripts of strictly identical nucleotide sequence in both pituitary and gonads, predicting a unique primary structure for the receptor protein. In addition, restriction analysis of rat genomic DNA suggested that a single gene encodes the mRNA subspecies identified to date and demonstrated the presence of introns included within the highly conserved coding region. Although post-translational modifications of the GnRH-receptor may differ in the various tissues examined, these data at least clarify the nature and identity of gonadal GnRH receptors. © 1994 Academic Press, Inc.
Authors & Co-Authors
Moumni, Mohieddine
France, Paris
Cnrs Centre National de la Recherche Scientifique
Morocco, Meknes
Université Moulay Ismaïl
Kottler, Marie Laure
France, Paris
Cnrs Centre National de la Recherche Scientifique
Counis, Raymond
France, Paris
Cnrs Centre National de la Recherche Scientifique
Statistics
Citations: 46
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.1006/bbrc.1994.1601
ISSN:
0006291X
Research Areas
Genetics And Genomics