Fabrication of thermodynamically stable self-microemulsifying drug delivery system of resveratrol with enhanced solubility and chemical stability

Resveratrol (RES) is a polyphenol and phytoalexin present in most of the herbal plants and its products. It is frequently prescribed as a preventing therapy for diseases like cancer, cardiovascular, neurodegenerative and many others. However, it is usually reported the problem of poor water solubility, poor bioavailability, and rapid metabolism, limits its further clinical applications. Self-microemulsifying drug delivery systems (SMEDDS) is an efficient technology that can be used to overcome these limitations. We have optimized and prepared Resveratrol SMEDDS formulations for the first time by using different concentrations of various oils, surfactants, and co-surfactant mixtures. These vehicles helped to construct pseudo ternary phase diagrams and yielded good microemulsion region. Optimized and most stable formulation was selected for further characterizations. Then, the final formulation was identified which was further taken out for stability study in different media and through accelerated stability studies. The results of the dissolution study indicated that the rate of dissolution was higher in comparison to the pure resveratrol. The droplet size of the formulation was less than 100 nm with negative charge. It indicates that the adsorption of negatively charged surfactant molecules at the interface of two phases of the microemulsion. Results of the MTT study showed that, more than 75% of the cells were visible in both the cell lines (HEK-293 & HEP-G2). It gives an indication of biosafety of the formulation. We have successfully synthesized highly solubilized and biocompatible formulation of RES.