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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Detection of a novel border disease virus subgroup in Tunisian sheep
Archives of Virology, Volume 150, No. 2, Year 2005
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Description
Nine pestiviruses isolated from different batches of a contaminated Tunisian sheep pox vaccine and one Tunisian field ovine isolate of border disease virus (BDV) were studied at the antigenic and molecular levels. Seroneutralization tests were carried out on three vaccine isolates, the Tunisian field isolate and representative reference strains of the different pestivirus groups. The antigenic study showed that the Tunisian isolates were closer to the two BDV reference strains than to the Alfort-187 and the NADL reference strains. Comparison of the nucleotide sequences of the 5′-non coding regions of all the Tunisian isolates to those of other pestiviruses have shown that these isolates were distinct from the established pestivirus species. The entire Npro-E2 coding sequences of four Tunisian isolates were determined and compared to other pestiviruses. Segregation of these pestiviruses based on the Npro-E2 region was identical to that obtained with the 5′UTR sequences. The phylogenetic tree obtained with these sequences showed that the Tunisian isolates formed a separate branch between the BDV and CSFV groups, and consequently a possible new species within the pestivirus genus. However, as indicated by the antigenic study and the host origin of the isolates, the Tunisian isolates were assigned to a novel subgroup within the BDV species. © Springer-Verlag 2004.
Authors & Co-Authors
Thabti, Fatma
France, Maisons-alfort
Afssa Agence Française de Sécurité Sanitaire Des Aliments
Tunisia, Tunis
Institut de Recherche Vétérinaire de Tunis
Letellier, Carine
Belgium, Brussels
Ctr. D'etud. Rech. Veterinaires A.
Hammami, Salah
Tunisia, Tunis
Institut de Recherche Vétérinaire de Tunis
Pépin, Michel
France, Maisons-alfort
Afssa Agence Française de Sécurité Sanitaire Des Aliments
Ribière, M.
Unknown Affiliation
Mesplède, A.
France, Maisons-alfort
Afssa Agence Française de Sécurité Sanitaire Des Aliments
Kerkhofs, P.
Belgium, Brussels
Ctr. D'etud. Rech. Veterinaires A.
Russo, Pierre
France, Maisons-alfort
Afssa Agence Française de Sécurité Sanitaire Des Aliments
Statistics
Citations: 71
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1007/s00705-004-0427-3
ISSN:
03048608