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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A simple and economical direct agglutination test for serodiagnosis and sero-epidemiological studies of visceral leishmaniasis
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 80, No. 4, Year 1986
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Description
A simple and economical direct agglutination test for the detection of visceral leishmaniasis is described. Trypsin-treated, Coomassie Brilliant Blue-stained, formalin-preserved promastigotes were used as antigen in re-usable V-well microtitre plates. In 21 patients with recent kala-azar, titres of 1:51200 or higher were found. Cured kala-azar patients treated 4 to 14 months before testing, showed titres in the range of 1:3,200 to >1:51,200. Healthy and diseased controls had titres below 1:1,600 with the exception of African trypanosomiasis patients who showed titres of 1:200 to 1:12,800, overlapping with the titres of cured kala-azar patients. Where trypanosomiasis is not a consideration, a titre of 1:1,600 could be considered indicative of visceral leishmaniasis, the sensitivity and specificity were then 100%. The test was applied to sera of 280 inhabitants of Baringo District, a known focus of visceral leishmaniasis in Kenya. When treated cases were included, the test showed a sensitivity of 100% and specificity of 99·3%. This test could be used in district hospitals and health centres in endemic areas as an aid in diagnosis of kala-azar and in the field for sero-epidemiological studies. © 1986, Oxford University Press.
Authors & Co-Authors
El-Harith, Abdallah
Netherlands, Amsterdam
Royal Tropical Institute - Kit
Kolk, Arend H.J.
Netherlands, Amsterdam
Royal Tropical Institute - Kit
Kager, Petrus A.
Netherlands, Amsterdam
Universiteit Van Amsterdam
Leeuwenburg, Johannis
Kenya, Nairobi
Kenya Medical Research Institute
Muigai, Richard K.
Kenya, Nairobi
Clinical Research Centre
Kiugu, S.
Kenya, Nairobi
Clinical Research Centre
Kiugu, S. K.
Kenya, Nairobi
Kenya Medical Research Institute
Laarman, Jacob J.
Netherlands, Amsterdam
Royal Tropical Institute - Kit
Statistics
Citations: 252
Authors: 8
Affiliations: 4
Identifiers
Doi:
10.1016/0035-9203(86)90149-5
ISSN:
00359203
e-ISSN:
18783503
Study Locations
Kenya