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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A rapid method to quantify pro-oxidant activity in cultures of wood-decaying white-rot fungi
Journal of Microbiological Methods, Volume 61, No. 2, Year 2005
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Description
A new, rapid method for evaluation of lipid peroxidation promoting (pro-oxidant) activity in cultures of wood-decaying fungi was developed. The method is based on measurement of the rate of oxygen consumption in the reaction of linoleic acid peroxidation initiated by fungal culture filtrates. The liquid cultures of the white-rot fungi Bjerkandera adusta and Phanerochaete chrysosporium grown on wheat straw-containing glucose-peptone-corn steep liquor medium possessed significant levels of the pro-oxidant activity. Other white-rot fungi producing manganese peroxidase (MnP) were also found to show the activity. MnP demonstrated a crucial role as the major pro-oxidant agent in the fungal cultures. The total pro-oxidant activity may be considered as net result of the peroxidation by MnP and the inhibition by antioxidant compounds present in the fungal culture fluids. © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Kapich, Alexander N.
Finland, Helsinki
Helsingin Yliopisto
South Africa, Stellenbosch
Stellenbosch University
Belarus, Minsk
International Sakharov Environmental Institute of Belarusian State University
Prior, Bernard Alexander
South Africa, Stellenbosch
Stellenbosch University
Lundell, Taina K.
Finland, Helsinki
Helsingin Yliopisto
Hatakka, Annele I.
Finland, Helsinki
Helsingin Yliopisto
Statistics
Citations: 22
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1016/j.mimet.2004.12.010
ISSN:
01677012