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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Herpesvirus detection and cytokine levels (IL-10, IL-6, and IFN-γ) in ocular fluid from Tunisian immunocompetent patients with uveitis
Journal of Medical Virology, Volume 85, No. 12, Year 2013
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Description
Human herpesviruses (HHVs) are involved in the pathogenesis of different types of uveitis. Cytokine response plays an important role in virus-induced immunopathology. This study aimed to investigate the incidence of HHVs in aqueous humor samples of immunocompetent patients with suspected viral uveitis and cytokine (IL-10, IL-6, and IFN-γ) expression profiling. Forty-seven aqueous humor samples were collected from immunocompetent patients with viral uveitis. Samples were assayed for HHV-1 to HHV-8 by in-house real-time polymerase chain reactions. IL-6, IL-10, and IFN-γ were quantified with a cytometric bead array. Relations between viral detection, cytokine profiles, and clinical data were studied. At least one viral genome was detected in 21 aqueous humor samples analyzed. Varicella-zoster virus (VZV) was detected in 14 of the positive samples, cytomegalovirus (CMV) in 8, HSV-1 in 1, Epstein-Barr virus (EBV) in 4, and HHV-6 in 2. More than one viral genome was detected in seven aqueous humor samples. Aqueous humor samples positive for HHV-DNA contained significant levels of IL-6, IL-10, and IFN-γ, compared to HHV-DNA negative samples. High levels of IL-6 were detected in patients with CMV-DNA in their aqueous humor samples. Significantly higher levels of IL-10 and IFN-γ were found in positive samples for VZV, EBV, and HHV-6 DNA than in negative aqueous humor ones. VZV was the principal etiologic agent of uveitis in this Tunisian series, with CMV the second most common agent. Knowledge of immunoregulatory interactions and dynamic changes in viral uveitis may be a key to understand the pathogenesis leading to more-effective treatments. J. Med. Virol. 85:2079-2086, 2013. © 2013 Wiley Periodicals, Inc.
Authors & Co-Authors
Nahdi, Imen
Tunisia, Monastir
Université de Monastir
Abdelwahed, Rym Ben
Tunisia, Monastir
Université de Monastir
France, Paris
Inserm
Boukoum, Hanen
Tunisia, Monastir
Université de Monastir
Bressollette-Bodin, Céline
France, Nantes
Nantes Université
Attia, Sonia
Tunisia, Ariana
Fattouma Bourguiba University Hospital
Ben-Yahia, Salim
Tunisia, Ariana
Fattouma Bourguiba University Hospital
Fisson, Sylvain
France, Paris
Inserm
France, Evry
Université D'evry Val D'essonne
Khairallah, Moncef
Tunisia, Ariana
Fattouma Bourguiba University Hospital
Aouni, Mahjoub
Tunisia, Monastir
Université de Monastir
Statistics
Citations: 11
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1002/jmv.23708
ISSN:
01466615
e-ISSN:
10969071
Research Areas
Genetics And Genomics
Study Design
Cohort Study