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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Lack of infection of vertebrate cells by the densovirus from the maize worm Mythimna loreyi (MlDNV)
Virus Research, Volume 99, No. 1, Year 2004
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Description
Several densoviruses have been used successfully in biological control of pests in the tropics. The densovirus from Mythimna loreyi (MlDNV) could also be an important tool in biological control of important pests. However, safety concerns remain as previous reports suggested that densoviruses may infect and transform L cells (from mouse). In this study, we show using molecular-biology tools that neither L nor other vertebrate cells support replication or transcription of densovirus, either after infection or after transfection. Quantitative PCR indicated no increase of viral DNA due to replication in vertebrate cells, in contrast to that in insect LD652 cells. After transfection, both the NS and VP mRNAs could be detected in LD652 cells but not in L cells. Moreover, the viral genome was excised from the plasmid after transfection of the infectious clone in LD652 cells, indicative of viral NS protein production, in contrast to L cells. The viral genome was able to integrate in the host chromosome of L cells after transfection, but not after infection. However, no viral transcription could be detected after integration. © 2003 Elsevier B.V. All rights reserved.
Authors & Co-Authors
El-Far, Mohamed
Canada, Quebec
Université Laval
Li, Yi
Canada, Quebec
Université Laval
Fédière, G.
Canada, Quebec
Université Laval
Egypt, Giza
Faculty of Agriculture
Abol-Ela, Said
Egypt, Giza
Faculty of Agriculture
Tijssen, Peter
Canada, Quebec
Université Laval
Statistics
Citations: 28
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1016/j.virusres.2003.09.010
ISSN:
01681702
Research Areas
Genetics And Genomics
Study Approach
Quantitative