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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Immunostimulatory effect of IL-18-encoding plasmid in DNA vaccination against murine Schistosoma mansoni infection
Vaccine, Volume 19, No. 11-12, Year 2001
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Description
In vivo delivery of DNA encoding antigens is a simple tool to induce immune responses against pathogens. This approach to vaccination also offers the possibility to codeliver plasmids encoding immunomodulatory molecules in order to drive immune responses towards optimal protective effects. In the murine model of Schistosoma mansoni infection, vaccination inducing a Th1 profile has been shown to be protective. In this study, we used a plasmid encoding the Th1-promoting cytokine IL-18, since we observed that percutaneous infection of Balb/c mice strongly induced the production of IL-18 mRNA in the skin. Intradermal injection of the IL-18-encoding plasmid prior to infection did not interfere with parasite migration through the skin although it led to a local and transient cellular infiltration. When the IL-18-encoding plasmid was codelivered with a S. mansoni glutathione S-transferase (Sm28GST)-encoding plasmid, a 30-fold increase of antigen-specific IFN-γ secretion by spleen cells was observed in comparison to spleen cells from mice that had received only the Sm28GST-encoding plasmid. This immunostimulatory effect was related to a significant protective effect (28% reduction in egg laying and 23% reduction in worm burden) which was attributed to a cooperative effect between both plasmids. Therefore, this study shows that codelivery of an IL-18-encoding plasmid with an antigen-encoding plasmid can stimulate specific cellular responses and induce protective effects against S. mansoni infection. © 2001 Elsevier Science Ltd.
Authors & Co-Authors
Dupré, Loï̈c
France, Paris
Inserm
Italy, Milan
San Raffaele Telethon Institute for Gene Therapy
Kremer, Laurent Daniel
France, Paris
Inserm
Wolowczuk, Isabelle
France, Paris
Cnrs Centre National de la Recherche Scientifique
Riveau, Gilles J.
France, Paris
Inserm
Caprón, André R.
France, Paris
Inserm
Locht, Camille
France, Paris
Inserm
Statistics
Citations: 46
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/S0264-410X(00)00363-7
ISSN:
0264410X
Research Areas
Genetics And Genomics