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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion
Malaria Journal, Volume 9, No. 1, Article 293, Year 2010
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Description
Background. Alternative arrangements of chromosome 2 inversions in Anopheles gambiae are important sources of population structure, and are associated with adaptation to environmental heterogeneity. The forces responsible for their origin and maintenance are incompletely understood. Molecular characterization of inversion breakpoints provides insight into how they arose, and provides the basis for development of molecular karyotyping methods useful in future studies. Methods. Sequence comparison of regions near the cytological breakpoints of 2Rb allowed the molecular delineation of breakpoint boundaries. Comparisons were made between the standard 2R+ b arrangement in the An. gambiae PEST reference genome and the inverted 2Rb arrangements in the An. gambiae M and S genome assemblies. Sequence differences between alternative 2Rb arrangements were exploited in the design of a PCR diagnostic assay, which was evaluated against the known chromosomal banding pattern of laboratory colonies and field-collected samples from Mali and Cameroon. Results. The breakpoints of the 7.55 Mb 2Rb inversion are flanked by extensive runs of the same short (72 bp) tandemly organized sequence, which was likely responsible for chromosomal breakage and rearrangement. Application of the molecular diagnostic assay suggested that 2Rb has a single common origin in An. gambiae and its sibling species, Anopheles arabiensis, and also that the standard arrangement (2R+b) may have arisen twice through breakpoint reuse. The molecular diagnostic was reliable when applied to laboratory colonies, but its accuracy was lower in natural populations. Conclusions. The complex repetitive sequence flanking the 2Rb breakpoint region may be prone to structural and sequence-level instability. The 2Rb molecular diagnostic has immediate application in studies based on laboratory colonies, but its usefulness in natural populations awaits development of complementary molecular tools. © 2010 Lobo et al; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2988034/bin/1475-2875-9-293-S1.PDF
Authors & Co-Authors
Lobo, Neil Francis
United States, Notre Dame
University of Notre Dame
Sangaré, Djibril D.
United States, Notre Dame
University of Notre Dame
Mali, Bamako
Malaria Research and Training Center
Regier, Allison A.
United States, Notre Dame
University of Notre Dame
Reidenbach, Kyanne R.
United States, Notre Dame
University of Notre Dame
Bretz, David A.
United States, Notre Dame
University of Notre Dame
Sharakhova, Maria V.
United States, Notre Dame
University of Notre Dame
United States, Blacksburg
Virginia Polytechnic Institute and State University
Emrich, Scott J.
United States, Notre Dame
University of Notre Dame
Traoré, Sékou Fantamady
Mali, Bamako
Malaria Research and Training Center
Costantini, Carlo
France, Montpellier
Ird Centre de Montpellier
Cameroon, Yaounde
Institut de Recherche Pour le Développement Ird, Organisation de Coordination Pour la Lutte Contre Les Endémies en Afrique Centrale Oceac
Besansky, Nora J.
United States, Notre Dame
University of Notre Dame
Collins, Frank H.
United States, Notre Dame
University of Notre Dame
Statistics
Citations: 42
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1186/1475-2875-9-293
e-ISSN:
14752875
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Cameroon
Mali