Voltammetric Determination of Nicotine at Poly(4-Amino-3-Hydroxynaphthalene Sulfonic Acid)-Modified Glassy Carbon Electrode

A poly(4-amino-3-hydroxynaphthalene sulfonic acid)-modified glassy carbon electrode was developed for the voltammetric determination of nicotine. An oxidation signal at lower potential with higher current was obtained for nicotine at the polymer-modified electrode compared to bare glassy carbon electrode suggesting the electrocatalytic effect of the polymer film. Under optimum conditions, square wave voltammetry of nicotine showed oxidation at +884mV (vs. SCE) in phosphate buffer solution pH7.5. The calibration curve was linear over the range 1.0 to 200.0μM (R 2=0.9987) concentration of nicotine with a detection limit (S/N=3) of 8.66×10 -7molL -1. Application of the proposed method to cigarette tobacco samples gave acceptable recovery results of 90.7% to 107.2%. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.