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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Morphogenesis of Bullet-Shaped Rabies Virus Particles Regulated by TSG101
Journal of Virology, Volume 97, No. 5, Year 2023
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Description
Viral protein assembly and virion budding are tightly regulated to enable the proper formation of progeny virions. At this late stage in the virus life cycle, some enveloped viruses take advantage of the host endosomal sorting complex required for transport (ESCRT) machinery, which contributes to the physiological functions of membrane modulation and abscission. Bullet-shaped viral particles are unique morphological characteristics of rhabdoviruses; however, the involvement of host factors in rhabdovirus infection and, specifically, the molecular mechanisms underlying virion formation are not fully understood. In the present study, we used a small interfering RNA (siRNA) screening approach and found that the ESCRT-I component TSG101 contributes to the propagation of rabies virus (RABV). We demonstrated that the matrix protein (M) of RABV interacts with TSG101 via the late domain containing the PY and YL motifs, which are conserved in various viral proteins. Loss of the YL motif in the RABV M or the downregulation of host TSG101 expression resulted in the intracellular aggregation of viral proteins and abnormal virus particle formation, indicating a defect in the RABV assembly and budding processes. These results indicate that the interaction of the RABV M and TSG101 is pivotal for not only the efficient budding of progeny RABV from infected cells but also for the bullet-shaped virion morphology. Copyright © 2023 Itakura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Authors & Co-Authors
Itakura, Yukari
Japan, Sapporo
Hokkaido University
Tabata, Koshiro
Japan, Sapporo
Hokkaido University
Kishimoto, Mai
Japan, Sapporo
Hokkaido University
Torii, Shiho
Japan, Sapporo
Hokkaido University
Kobayashi, Shintaro
Japan, Sapporo
Hokkaido University
Ito, Naoto
Japan, Gifu
Gifu University
Maeda, Ken
Japan, Tokyo
National Institute of Infectious Diseases
Takada, Ayato
Japan, Sapporo
Hokkaido University
Hall, William W.
Ireland, Dublin
University College Dublin
Japan, Sapporo
Hokkaido University
United States, Baltimore
Global Virus Network
Orba, Yasuko
Japan, Sapporo
Hokkaido University
Sawa, Hirofumi
Japan, Sapporo
Hokkaido University
United States, Baltimore
Global Virus Network
Sasaki, Michihito
Japan, Sapporo
Hokkaido University
Statistics
Citations: 1
Authors: 12
Affiliations: 5
Identifiers
Doi:
10.1128/jvi.00438-23
ISSN:
0022538X
Research Areas
Health System And Policy