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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Comparison of preservation methods of Rhipicephalus appendiculatus (Acari: Ixodidae) for reliable DNA amplification by PCR
Experimental and Applied Acarology, Volume 38, No. 2-3, Year 2006
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Description
Five differently preserved groups of adult Rhipicephalus appendiculatus specimens were compared for quality of DNA extracted. Three methods were used to extract DNA from specimens i.e. two simple mosquito validated DNA extraction methods and a tick validated method. Extraction of DNA from tick legs was attempted. The quality of DNA extracted was evaluated by the success of PCR amplification of the ITS2 gene and the mitochondrial COI gene fragment. Fresh specimens (i.e. killed just before extraction) had the highest success of DNA amplification followed by specimens killed in ethanol and subsequently stored in the refrigerator (4°C). There was no significant difference in amplification success between cryopreserved and 70% ethanol preserved specimens. It was possible to amplify DNA from legs of ticks. Sequenced ITS2 amplicon of template obtained from legs of ticks was as legible as those from whole tick extract. The two mosquito validated DNA extraction methods showed a significantly lower amplification success than the tick validated protocol. © Springer 2006.
Authors & Co-Authors
Mtambo, Jupiter
Unknown Affiliation
Van Bortel, Wim
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Madder, Maxime
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Roelants, Patricia
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Backeljau, Thierry
Belgium, Brussels
Koninklijk Belgisch Instituut Voor Natuurwetenschappen
Statistics
Citations: 22
Authors: 5
Affiliations: 2
Identifiers
Doi:
10.1007/s10493-006-0004-4
ISSN:
15729702
Research Areas
Genetics And Genomics