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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
Matrix-Assisted Laser Desorption Ionization - Time of Flight Mass Spectrometry: An Emerging Tool for the Rapid Identification of Mosquito Vectors
PLoS ONE, Volume 8, No. 8, Article e72380, Year 2013
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Description
Background:The identification of mosquito vectors is typically based on morphological characteristics using morphological keys of determination, which requires entomological expertise and training. The use of protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), which is increasingly being used for the routine identification of bacteria, has recently emerged for arthropod identification.Methods:To investigate the usefulness of MALDI-TOF-MS as a mosquito identification tool, we tested protein extracts made from mosquito legs to create a database of reference spectra. The database included a total of 129 laboratory-reared and field-caught mosquito specimens consisting of 20 species, including 4 Aedes spp., 9 Anopheles spp., 4 Culex spp., Lutzia tigripes, Orthopodomyia reunionensis and Mansonia uniformis. For the validation study, blind tests were performed with 76 specimens consisting of 1 to 4 individuals per species. A cluster analysis was carried out using the MALDI-Biotyper and some spectra from all mosquito species tested.Results:Biomarker mass sets containing 22 and 43 masses have been detected from 100 specimens of the Anopheles, Aedes and Culex species. By carrying out 3 blind tests, we achieved the identification of mosquito vectors at the species level, including the differentiation of An. gambiae complex, which is possible using MALDI-TOF-MS with 1.8 as the cut-off identification score. A cluster analysis performed with all available mosquito species showed that MALDI-Biotyper can distinguish between specimens at the subspecies level, as demonstrated for An gambiae M and S, but this method cannot yet be considered a reliable tool for the phylogenetic study of mosquito species.Conclusions:We confirmed that even without any specific expertise, MALDI-TOF-MS profiling of mosquito leg protein extracts can be used for the rapid identification of mosquito vectors. Therefore, MALDI-TOF-MS is an alternative, efficient and inexpensive tool that can accurately identify mosquitoes collected in the field during entomological surveys. © 2013 Yssouf et al.
Authors & Co-Authors
Yssouf, Amina
France, Paris
Inserm
Socolovschi, Cristina
France, Paris
Inserm
Flaudrops, Christophe
France, Marseille
Ap-hm Assistance Publique - Hôpitaux de Marseille
Ndiath, Mamadou Ousmane
France, Paris
Inserm
Sougoufara, Seynabou
France, Paris
Inserm
DeHecq, Jean Sébastien
Unknown Affiliation
Lacour, Guillaume
France, Montpellier
Entente Interdépartementale Pour la Démoustication du Littoral Méditerranéen
Bérenger, Jean Michel
France, Paris
Inserm
Sokhna, Cheikh Sadibou
France, Paris
Inserm
Raoult, Didier A.
France, Paris
Inserm
Parola, Philippe
France, Paris
Inserm
Statistics
Citations: 98
Authors: 11
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0072380
ISSN:
19326203
Research Areas
Disability
Study Design
Cross Sectional Study