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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Beryllium binding to HLA-DP molecule carrying the marker of susceptibility to berylliosis glutamate β69
Human Immunology, Volume 62, No. 7, Year 2001
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Description
Berylliosis is a chronic granulomatous disorder caused by inhalation of Be dusts that is driven by the accumulation Be-specific CD4+ Th1-cells at disease sites. Susceptibility to berylliosis has been associated with the supratypic variant of HLA-DP gene coding for glutamate at position β69 (HLA-DPβGlu69). The aim of this study was to test the hypothesis that the HLA-DPβGlu69 residue plays a role in the interaction with Be. To this end, soluble HLA-DP2 molecule (carrying βGlu69) and its mutated form carrying lysine at position β69 (HLA-DP2Lys69) were produced in Drosophila melanogaster and then used in a Be binding assays. BeSO4 (1-1000 μM) was used to compete for the binding of the biotinilated invariant chain-derived peptide CLIP (50 μM). BeSO4 was capable of compete out biotin-CLIP binding from the HLA-DP2 (IC50%: 4.5 μM of BeSO4 at pH 5.0 and 5.5 μM of BeSO4 at pH 7.5), but not from the HLA-DP2Lys69 molecule (IC50%: 480 μM of BeSO4 at pH 5.0 and 220 μM of BeSO4 at pH 7.5). Moreover, the binding of NFLD.M60, a MoAb recognizing an epitope in the HLA-DP peptide binding region, to the HLA-DP2, but not to the HLA-DP2I.ys69 soluble molecules was inhibited BeSO4. NFLD.M60 binding to HLA-DP2, but not to HLA-DP2Lys69 stably transfected murine cells was also inhibited by Be both at pH 5.0 and at pH 7.5. The data indicate a direct interaction of Be with the HLA-DPGlu69 molecule, in the absence of antigen processing. © American Society for Histocompatibility and Immunogenetics, 2001.
Authors & Co-Authors
Amicosante, Massimo
Italy, Rome
Irccs Istituto Nazionale Malattie Infettive Lazzaro Spallanzani
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Sanarico, Nunzia
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Italy, Rome
Irccs Istituto Nazionale Malattie Infettive Lazzaro Spallanzani
Lechler, Robert Ian
United Kingdom, London
Imperial College London
Colizzi, Vittorio C.
Italy, Rome
Università Degli Studi Di Roma Tor Vergata
Italy, Rome
Irccs Istituto Nazionale Malattie Infettive Lazzaro Spallanzani
Saltini, Cesare
Italy, Rome
Irccs Istituto Nazionale Malattie Infettive Lazzaro Spallanzani
Statistics
Citations: 56
Authors: 5
Affiliations: 4
Identifiers
Doi:
10.1016/S0198-8859(01)00261-0
ISSN:
01988859
Research Areas
Genetics And Genomics