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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Enhanced detection and typing of human papillomavirus (HPV) DNA in anogenital samples with PGMY primers and the linear array HPV genotyping test
Journal of Clinical Microbiology, Volume 44, No. 6, Year 2006
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Description
The Roche PGMY primer-based research prototype line blot assay (PGMY-LB) is a convenient tool in epidemiological studies for the detection and typing of human papillomavirus (HPV) DNA. This assay has been optimized and is being commercialized as the Linear Array HPV genotyping test (LA-HPV). We assessed the agreement between LA-HPV and PGMY-LB for detection and typing of 37 HPV genotypes in 528 anogenital samples (236 anal, 146 physician-collected cervical, and 146 self-collected cervicovaginal swabs) obtained from human immunodeficiency virus-seropositive individuals (236 men and 146 women). HPV DNA was detected in 433 (82.0%) and 458 (86.7%) samples with PGMY-LB and LA-HPV (P = 0.047), respectively, for an excellent agreement of 93.8% (kappa = 0.76). Of the 17,094 HPV typing results, 16,562 (1,743 positive and 14,819 negative results) were concordant between tests (agreement = 96.9%; kappa = 0.76). The mean agreement between tests for each type was 96.4% ± 2.4% (95% confidence interval [CI], 95.6% to 97.2%; range, 86% to 100%), for an excellent mean kappa value of 0.85 ± 0.10 (95% CI, 0.82 to 0.87). However, detection rates for most HPV types were greater with LA-HPV. The mean number of types per sample detected by LA-HPV (4.2 ± 3.4; 95% CI, 3.9 to 4.5; median, 3.0) was greater than that for PGMY-LB (3.4 ± 3.0; 95% CI, 3.1 to 3.6; median, 2.0) (P < 0.001). The number of types detected in excess by LA-HPV in anal samples correlated with the number of types per sample (r = 0.49 ± 0.06; P = 0.001) but not with patient age (r = 0.03 ± 0.06; P = 0.57), CD4 cell counts (r = 0.06 ± 0.06; P = 0.13), or the grade of anal disease (r = -0.11 ± 0.06; P = 0.07). LA-HPV compared favorably with PGMY-LB but yielded higher detection rates for newer and well-known HPV types. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Coutlée, François
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Canada, Montreal
University of Montreal
Canada, Montreal
Université Mcgill
Rouleau, Danielle
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Canada, Montreal
University of Montreal
Petignat, P.
Canada, Montreal
Centre Hospitalier de L'universite de Montreal
Kornegay, Janet R.
United States, Pleasanton
Roche Molecular Systems
Hankins, Catherine
Canada, Montreal
Université Mcgill
Canada
Gouvernement du Québec
Walmsley, Sharon Lynn
Canada, Toronto
Toronto General Hospital
Franco, Eduardo Luis Fabiano
Canada, Montreal
Université Mcgill
Haase, David A.
Unknown Affiliation
Smaill, Fiona Mary
Unknown Affiliation
Falutz, Julian M.
Unknown Affiliation
Ferenczy, Alex S.
Unknown Affiliation
Klein, Marina Barbara
Unknown Affiliation
Labrecque, Louise Geneviève
Unknown Affiliation
Routy, Jean Pierre G.
Unknown Affiliation
Trottier, Sylvie
Unknown Affiliation
Rachlis, Anita Rochelle
Unknown Affiliation
Braitstein, Paula K.A.
Unknown Affiliation
Burdge, David R.
Unknown Affiliation
Harris, Marianne
Unknown Affiliation
Money, Deborah M.
Unknown Affiliation
Montaner, Julio S.G.
Unknown Affiliation
Statistics
Citations: 151
Authors: 21
Affiliations: 7
Identifiers
Doi:
10.1128/JCM.00104-06
ISSN:
00951137
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Participants Gender
Male
Female