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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
The tetherin antagonism of the Ebola virus glycoprotein requires an intact receptor-binding domain and can be blocked by GP1-specific antibodies
Journal of Virology, Volume 90, No. 24, Year 2016
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Description
The glycoprotein of Ebola virus (EBOV GP), a member of the family Filoviridae, facilitates viral entry into target cells. In addition, EBOV GP antagonizes the antiviral activity of the host cell protein tetherin, which may otherwise restrict EBOV release from infected cells. However, it is unclear how EBOV GP antagonizes tetherin, and it is unknown whether the GP of Lloviu virus (LLOV), a filovirus found in dead bats in Northern Spain, also counteracts tetherin. Here, we show that LLOV GP antagonizes tetherin, indicating that tetherin may not impede LLOV spread in human cells. Moreover, we demonstrate that appropriate processing of N-glycans in tetherin/GP-coexpressing cells is required for tetherin counteraction by EBOV GP. Furthermore, we show that an intact receptor-binding domain (RBD) in the GP1 subunit of EBOV GP is a prerequisite for tetherin counteraction. In contrast, blockade of Niemann-Pick disease type C1 (NPC1), a cellular binding partner of the RBD, did not interfere with tetherin antagonism. Finally, we provide evidence that an antibody directed against GP1, which protects mice from a lethal EBOV challenge, may block GP-dependent tetherin antagonism. Our data, in conjunction with previous reports, indicate that tetherin antagonism is conserved among the GPs of all known filoviruses and demonstrate that the GP1 subunit of EBOV GP plays a central role in tetherin antagonism. © 2016, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Qiu, Xiangguo
Canada, Ottawa
Public Health Agency of Canada
Takada, Ayato
Japan, Sapporo
Hokkaido University
Schindler, Michael
Germany, Tubingen
Universitätsklinikum Und Medizinische Fakultät Tübingen
Germany, Oberschleissheim
Helmholtz Center Munich German Research Center for Environmental Health
Pöhlmann, Stefan H.
Germany, Gottingen
Deutsches Primatenzentrum
Statistics
Citations: 18
Authors: 4
Affiliations: 6
Identifiers
Doi:
10.1128/JVI.01563-16
ISSN:
0022538X
Research Areas
Infectious Diseases