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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor
Molecular Endocrinology, Volume 6, No. 7, Year 1992
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Description
GnRH plays a pivotal role in the reproductive system, and GnRH analogs have wide therapeutic applications ranging from the treatment of prostatic cancer to infertility. Determination of the predicted structure of the GnRH receptor (GnRHR) would illuminate the mechanisms of receptor activation and regulation and allow directed design of improved GnRH analogs. We report the cloning of a cDNA representing the mouse GnRHR and confirm its identity using Xenopus oocyte expression. Injection of sense RNA transcript leads to the expression of a functional, high affinity GnRHR. Expression of the GnRHR using gonadotrope cell line RNA, however, is blocked by an antisense oligonucleotide. In situ hybridization in the rat anterior pituitary reveals a characteristic GnRHR distribution. The nucleotide sequence encodes a 327-amino acid protein which has the seven putative transmembrane domains characteristic of G protein-coupled receptors, but which lacks a typical intracellular C-terminus. The unusual structure and novel potential regulatory domain of the GnRHR may explain unique aspects of its signal transduction and regulation. © 1992 by The Endocrine Society.
Authors & Co-Authors
Tsutsumi, Manami
United States, New York
Icahn School of Medicine at Mount Sinai
Zhou, Wei
United States, New York
Icahn School of Medicine at Mount Sinai
Millar, Robert P.
South Africa, Cape Town
Faculty of Health Sciences
Mellon, Pamela L.
United States, La Jolla
University of California, San Diego
Roberts, James Lewis
United States, New York
Icahn School of Medicine at Mount Sinai
Flanagan, Colleen A.
South Africa, Cape Town
Faculty of Health Sciences
Dong, Kewen
United States, New York
Icahn School of Medicine at Mount Sinai
Gillo, Boaz
United States, New York
Icahn School of Medicine at Mount Sinai
Sealfon, Stuart C.
United States, New York
Icahn School of Medicine at Mount Sinai
Statistics
Citations: 356
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1210/mend.6.7.1324422
ISSN:
08888809
e-ISSN:
19449917
Research Areas
Cancer
Genetics And Genomics
Sexual And Reproductive Health