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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Genome Sequence of Lactobacillus pentosus KCA1: Vaginal Isolate from a Healthy Premenopausal Woman
PLoS ONE, Volume 8, No. 3, Article e59239, Year 2013
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Description
The vaginal microbiota, in particular Lactobacillus species, play an important role in female health through modulation of immunity, countering pathogens and maintaining a pH below 4.7. We report the isolation and genome sequence of Lactobacillus pentosus strain KCA1 (formally known as L. plantarum) from the vagina of a healthy Nigerian woman. The genome was sequenced using Illumina GA II technology. The resulting 16,920,226 paired-end reads were assembled with the Velvet tool. Contigs were annotated using the RAST server, and manually curated. A comparative analysis with the available genomes of L. pentosus IG1 and L. plantarum WCFS1 showed that over 15% of the predicted functional activities are found only in this strain. The strain has a chromosome sequence of 3,418,159 bp with a G+C content of 46.4%, and is devoid of plasmids. Novel gene clusters or variants of known genes relative to the reference genomes were found. In particular, the strain has loci encoding additional putative mannose phosphotransferase systems. Clusters of genes include those for utilization of hydantoin, isopropylmalate, malonate, rhamnosides, and genes for assimilation of polyglycans, suggesting the metabolic versatility of L. pentosus KCA1. Loci encoding putative phage defense systems were also found including clustered regularly interspaced short palindromic repeats (CRISPRs), abortive infection (Abi) systems and toxin-antitoxin systems (TA). A putative cluster of genes for biosynthesis of a cyclic bacteriocin precursor, here designated as pentocin KCA1 (penA) were identified. These findings add crucial information for understanding the genomic and geographic diversity of vaginal lactobacilli. © 2013 Anukam et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s001.tiff
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s002.tiff
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s003.tiff
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s004.tiff
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s005.tiff
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s006.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s007.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s008.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s009.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC3602190/bin/pone.0059239.s010.docx
Authors & Co-Authors
Anukam, Kingsley
Nigeria, Benin
University of Benin
Canada, London
Lawson Health Research Institute
Macklaim, Jean M.
Canada, London
Lawson Health Research Institute
Canada, London
Western University
Gloor, Gregory B.
Canada, London
Lawson Health Research Institute
Canada, London
Western University
Reid, Gregor
Canada, London
Lawson Health Research Institute
Canada, London
Western University
Boekhorst, Jos
Netherlands, Nijmegen
Radboud University Medical Center
Netherlands, Wageningen
Ti Food and Nutrition
Netherlands, Nijmegen
Netherlands Bioinformatics Centre - Nbic
Renckens, Bernadet
Netherlands, Nijmegen
Radboud University Medical Center
Netherlands, Wageningen
Ti Food and Nutrition
Netherlands, Nijmegen
Netherlands Bioinformatics Centre - Nbic
van Hijum, Sacha A.F.T.
Netherlands, Nijmegen
Radboud University Medical Center
Netherlands, Wageningen
Ti Food and Nutrition
Netherlands, Nijmegen
Netherlands Bioinformatics Centre - Nbic
Siezen, Roland J.
Netherlands, Nijmegen
Radboud University Medical Center
Netherlands, Wageningen
Ti Food and Nutrition
Netherlands, Nijmegen
Netherlands Bioinformatics Centre - Nbic
Netherlands, Ede
Microbial Bioinformatics
Statistics
Citations: 60
Authors: 8
Affiliations: 7
Identifiers
Doi:
10.1371/journal.pone.0059239
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Noncommunicable Diseases
Participants Gender
Female