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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
engineering
Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa
Sensors and Actuators, B: Chemical, Volume 122, No. 2, Year 2007
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Description
We describe herein a newly developed optical immunosensor for detection of antibodies directed against antigens of the Ebola virus strains Zaire and Sudan. We employed a photo immobilization methodology based on a photoactivatable electrogenerated poly(pyrrole-benzophenone) film deposited upon an indium tin oxide (ITO) modified conductive surface fiber-optic. It was then linked to a biological receptor, Ebola virus antigen in this case, on the fiber tip through a light driven reaction. The photochemically modified optical fibers were tested as an immunosensor for detection of antibodies against Ebola virus, in animal and human sera, by use of a coupled chemiluminescent reaction. The immunosensor was tested for sensitivity, specificity, and compared to standard chemiluminescent ELISA under the same conditions. The analyte, anti-Ebola IgG, was detected at a low titer of 1:960,000 and 1:1,000,000 for subtypes Zaire and Sudan, respectively. While the same serum tested by ELISA was one order (24 times) less sensitive. © 2006 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Petrosova, A.
Israel, Beer-sheva
Ben-gurion University of the Negev
Konry, T.
Israel, Beer-sheva
Ben-gurion University of the Negev
Cosnier, Serge
France, Saint Martin D'heres
Université Grenoble Alpes
Trakht, I.
United States, New York
Columbia University
Lutwama, Julius Julian
Uganda, Entebbe
Uganda Virus Research Institute
Rwaguma, Elly B.
Uganda, Entebbe
Uganda Virus Research Institute
Chepurnov, A.
Russian Federation, Novosibirsk
Bsl-4 Laboratory
Mühlberger, Elke
Germany, Marburg
Philipps-universität Marburg
Lobel, Leslie I.
Israel, Beer-sheva
Ben-gurion University of the Negev
Marks, Robert S.
Israel, Beer-sheva
Ben-gurion University of the Negev
Statistics
Citations: 46
Authors: 10
Affiliations: 6
Identifiers
Doi:
10.1016/j.snb.2006.07.005
ISSN:
09254005
Research Areas
Infectious Diseases
Study Locations
Sudan