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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Molecular evidence for the localization of plasmodium falciparum immature gametocytes in bone marrow
Blood, Volume 123, No. 7, Year 2014
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Description
Plasmodium falciparum immature gametocytes are not observed in peripheral blood. However, gametocyte stages in organs such as bone marrow have never been assessed bymolecular techniques, which are more sensitive than optical microscopy. We quantified P falciparum sexual stages in bone marrow (n = 174) and peripheral blood (n = 70) of Mozambican anemic children by quantitative polymerase chain reaction targeting transcripts specific for early (PF14-0748; PHISTa), intermediate (PF13-0247; Pfs48/45), and mature (PF10-0303; Pfs25) gametocytes. Among children positive for the P falciparum housekeeping gene (PF08-0085; ubiquitin-conjugating enzyme gene) in bone marrow (n = 136) and peripheral blood (n = 25), prevalence of immature gametocytes was higher in bone marrow than peripheral blood (early: 95% vs 20%, P < .001; intermediate: 80% vs 16%; P < .001), as were transcript levels (P < .001 for both stages). In contrast, mature gametocytes were more prevalent (100% vs 51%, P < .001) and abundant (P < .001) in peripheral blood than in the bone marrow. Severe anemia (3.57, 95% confidence interval 1.49-8.53) and dyserythropoiesis (6.21, 95% confidence interval 2.24-17.25) were independently associated with a higher prevalence of mature gametocytes in bone marrow. Our results highlight the high prevalence and abundance of early sexual stages in bone marrow, aswell as the relationship between hematological disturbances and gametocyte development in this tissue. © 2014 by The American Society of Hematology.
Authors & Co-Authors
Aguilar, Ruth
Spain, Barcelona
Universitat de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Magallón-Tejada, Ariel
Spain, Barcelona
Universitat de Barcelona
Achtman, Ariel H.
Australia, Melbourne
Walter and Eliza Hall Institute of Medical Research
Australia, Melbourne
University of Melbourne
Moraleda, Cinta
Spain, Barcelona
Universitat de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Joice, Regina
United States, Boston
Harvard T.h. Chan School of Public Health
Cisteró, Pau
Spain, Barcelona
Universitat de Barcelona
Li-Wai-Suen, Connie S.N.
Australia, Melbourne
Walter and Eliza Hall Institute of Medical Research
Australia, Melbourne
University of Melbourne
Nhabomba, Augusto J.
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
MacEte, Eusébio V.
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Mueller, Ivo
Spain, Barcelona
Universitat de Barcelona
Australia, Melbourne
Walter and Eliza Hall Institute of Medical Research
Australia, Melbourne
University of Melbourne
Papua new Guinea, Goroka
Papua new Guinea Institute of Medical Research
Marti, Matthias
United States, Boston
Harvard T.h. Chan School of Public Health
Alonso, Pedro Luís
Spain, Barcelona
Universitat de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Menéndez, Clara
Spain, Barcelona
Universitat de Barcelona
Spain, Madrid
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Schofield, Louis
Australia, Melbourne
Walter and Eliza Hall Institute of Medical Research
Australia, Melbourne
University of Melbourne
Mayor, A. G.
Spain, Barcelona
Universitat de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Statistics
Citations: 146
Authors: 15
Affiliations: 7
Identifiers
Doi:
10.1182/blood-2013-08-520767
ISSN:
00064971
Research Areas
Genetics And Genomics
Maternal And Child Health
Sexual And Reproductive Health
Study Design
Cross Sectional Study
Study Approach
Quantitative