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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Potent action of RFamide-related peptide-3 on pituitary gonadotropes indicative of a hypophysiotropic role in the negative regulation of gonadotropin secretion
Endocrinology, Volume 149, No. 11, Year 2008
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Description
We identified a gene in the ovine hypothalamus encoding for RFamide-related peptide-3 (RFRP-3), and tested the hypothesis that this system produces a hypophysiotropic hormone that inhibits the function of pituitary gonadotropes. The RFRP-3 gene encodes for a peptide that appears identical to human RFRP-3 homolog. Using an antiserum raised against RFRP-3, cells were localized to the dorsomedial hypothalamic nucleus/paraventricular nucleus of the ovine brain and shown to project to the neurosecretory zone of the ovine median eminence, predicating a role for this peptide in the regulation of anterior pituitary gland function. Ovine RFRP-3 peptide was tested for biological activity in vitro and in vivo, and was shown to reduce LH and FSH secretion in a specific manner. RFRP-3 potently inhibited GnRH-stimulated mobilization of intracellular calcium in gonadotropes. These data indicate that RFRP-3 is a specific and potent mammalian gonadotropin-inhibiting hormone, and that it acts upon pituitary gonadotropes to reduce GnRH-stimulated gonadotropin secretion. Copyright © 2008 by The Endocrine Society.
Authors & Co-Authors
Clarke, Iain J.
Australia, Clayton
Monash University
Sari, Ika Puspita
Australia, Clayton
Monash University
Qi, Yue
Australia, Clayton
Monash University
Smith, Jeremy T.
Australia, Clayton
Monash University
Parkington, Helena C.
Australia, Clayton
Monash University
Ubuka, Takayoshi
United States, Berkeley
University of California, Berkeley
Iqbal, Javed
Australia, Clayton
Monash University
Li, Qun
Australia, Clayton
Monash University
Tilbrook, Alan
Australia, Clayton
Monash University
Morgan, Kevin
United Kingdom, Edinburgh
University of Edinburgh, College of Medicine and Veterinary Medicine
Pawson, Adam J.
United Kingdom, Edinburgh
University of Edinburgh, College of Medicine and Veterinary Medicine
Tsutsui, Kazuyoshi
Japan, Tokyo
Waseda University
Millar, Robert P.
United Kingdom, Edinburgh
University of Edinburgh, College of Medicine and Veterinary Medicine
South Africa, Cape Town
University of Cape Town
Bentley, George E.
United States, Berkeley
University of California, Berkeley
Statistics
Citations: 315
Authors: 14
Affiliations: 5
Identifiers
Doi:
10.1210/en.2008-0575
ISSN:
00137227
e-ISSN:
00137227
Research Areas
Genetics And Genomics