Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A prime-boost immunisation regimen using recombinant BCG and Pr55
gag
virus-like particle vaccines based on HIV type 1 subtype C successfully elicits Gag-specific responses in baboons
Vaccine, Volume 27, No. 35, Year 2009
Notification
URL copied to clipboard!
Description
Mycobacterium bovis BCG is considered an attractive live bacterial vaccine vector. In this study, we investigated the immune response of baboons to a primary vaccination with recombinant BCG (rBCG) constructs expressing the gag gene from a South African HIV-1 subtype C isolate, and a boost with HIV-1 subtype C Pr55gag virus-like particles (Gag VLPs). Using an interferon enzyme-linked immunospot assay, we show that although these rBCG induced only a weak or an undetectable HIV-1 Gag-specific response on their own, they efficiently primed for a Gag VLP boost, which strengthened and broadened the immune responses. These responses were predominantly CD8+ T cell-mediated and recognised similar epitopes as those targeted by humans with early HIV-1 subtype C infection. In addition, a Gag-specific humoral response was elicited. These data support the development of HIV-1 vaccines based on rBCG and Pr55gag VLPs. © 2009 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Chege, Gerald K.
South Africa, Cape Town
Faculty of Health Sciences
Kenya, Nairobi
National Museums of Kenya
Thomas, Robin
South Africa, Cape Town
Faculty of Health Sciences
Shephard, Enid G.
South Africa, Cape Town
Faculty of Health Sciences
Meyers, Ann E.
South Africa, Cape Town
Faculty of Health Sciences
Bourn, William R.
South Africa, Cape Town
Faculty of Health Sciences
Williamson, Carolyn
South Africa, Cape Town
Faculty of Health Sciences
Maclean, James M.
South Africa, Cape Town
Faculty of Health Sciences
Gray, Clive M.
South Africa, Johannesburg
National Institute for Communicable Diseases
Rybicki, Edward P.
South Africa, Cape Town
Faculty of Health Sciences
Williamson, Anna Lise
South Africa, Cape Town
Faculty of Health Sciences
South Africa, Observatory
Groote Schuur Hospital
Statistics
Citations: 41
Authors: 10
Affiliations: 4
Identifiers
Doi:
10.1016/j.vaccine.2009.05.064
ISSN:
0264410X
Research Areas
Genetics And Genomics
Infectious Diseases