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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
High specificity of V3 serotyping among human immunodeficiency virus type-1 subtype C infected patients with varying disease status and viral phenotype
Journal of Medical Virology, Volume 78, No. 10, Year 2006
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Description
V3 serotyping is a technique for determining HIV-1 genetic subtype based on the binding of antibodies from patient sera or plasma to synthetic V3 peptides derived from subtype consensus sequences. Variation in the performance of this assay has been attributed to V3 sequence heterogeneity, the degree of which varies with patient disease progression, virus co-receptor usage, and genetic subtype. This study assessed the performance of a competitive peptide enzyme immunoassay (cPEIA) in samples from HIV-1 subtype C infected patients with varying disease profiles, including those with syncytium (SI) and non-syncytium-inducing (NSI) viruses. Out of 90 sera tested, 94.4% reacted strongly against the subtype C peptide. There was no significant difference in assay sensitivity among samples from advanced AIDS patients in which humoral immune response may be lower, nor among SI viruses which carry changes in the V3 sequence. Four samples were found to be cross-reactive with other subtypes and one acutely infected patient sample was non-reactive due to low anti-gp120 antibody titers. A significantly higher number of samples showed secondary reactivity to subtype A, compared to other subtypes (P < 0.005). In conclusion, the assay was able to identify HIV-1 subtype C infection with a high level of sensitivity (94%) irrespective of the stage of disease and therefore provides a valuable resource for the large-scale epidemiological monitoring of the spread of HIV-1 subtypes in South Africa. © 2006 Wiley-Liss, Inc.
Authors & Co-Authors
Walker, Polly R.
United Kingdom, Oxford
University of Oxford
South Africa, Johannesburg
National Institute for Communicable Diseases
Cilliers, Tonie
South Africa, Johannesburg
National Institute for Communicable Diseases
Choge, Isaac A.
South Africa, Johannesburg
National Institute for Communicable Diseases
Taylor, Natasha
South Africa, Johannesburg
National Institute for Communicable Diseases
Cohen, Sarah
South Africa, Johannesburg
National Institute for Communicable Diseases
Morris, Lynn
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 7
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1002/jmv.20690
ISSN:
01466615
e-ISSN:
10969071
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Locations
South Africa