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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Prevalence of toxoplasma gondii from free-range chickens (Gallus domesticus) from Addis Ababa, Ethiopia
Journal of Parasitology, Volume 99, No. 4, Year 2013
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Description
Prevalence of Toxoplasma gondii in free-range chickens (Gallus domesticus) is a good indicator of the environmental contamination with oocysts because chickens become infected mainly by feeding from ground, feed, or soil contaminated with oocysts. The seroprevalence of T. gondii antibodies in 125 free-range chickens from the Addis Ababa, Ethiopia, was determined. Antibodies to T. gondii were assayed by the modified agglutination test; 48 of 125 (38.4%) chickens were seropositive, with titers of 1:5 in 14, 1:10 in 12, 1:20 in 14, 1: 40 in 3, 1: 80 in 1, 1:160 in 1, 1:320 in 1, and ≥1:640 in 2 chickens. The hearts of 115 chickens were bioassayed for T. gondii infection. Hearts of 72 seronegative (modified agglutination test [MAT] < 1:5) chickens were pooled in 4 groups (20 + 18 + 19 + 15) and fed to 4 T. gondii-free cats; none of these 4 cats shed oocysts in their feces examined 3-21 days after feeding chicken tissues. Hearts of 43 seropositive chickens (MAT ≥ 1:5) were bioassayed individually in mice. Toxoplasma gondii was isolated from only 1 chicken, with a MAT titer of 1:80. This isolate was designated TgCKEt1 and was not pathogenic for outbred mice. Restricted fragment length polymorphism (RFLP) genotyping using 10 loci indicated the TgCKEt1 was ToxoDB polymerase chain reaction-RFLP genotype num1 (Type II clonal). Results of this study indicate very low environmental contamination with T. gondii oocysts around Addis Ababa. © American Society of Parasitologists 2013.
Authors & Co-Authors
Tilahun, Getachew Teshome
Ethiopia, Addis Ababa
Addis Ababa University
Tiao, Narry
United States, Columbus
The Ohio State University
Ferreira, Leandra Ribeiro
United States, Knoxville
The University of Tennessee, Knoxville
Choudhary, Shanti
United States, Knoxville
The University of Tennessee, Knoxville
Oliveira, Solange D.
United States, Knoxville
The University of Tennessee, Knoxville
Verma, Shiv Kumar
United States, Knoxville
The University of Tennessee, Knoxville
Kwok, Oliver Chun Hung
United States, Knoxville
The University of Tennessee, Knoxville
Molla, Bayleyegn Z.
United States, Columbus
The Ohio State University
Saville, William J.A.
United States, Columbus
The Ohio State University
Medhin, Girmay
Ethiopia, Addis Ababa
Addis Ababa University
Kassa, Tesfaye
Ethiopia, Addis Ababa
Addis Ababa University
Aleme, H.
Ethiopia, Addis Ababa
Addis Ababa University
Gebreyes, Wondwossen Abebe
United States, Columbus
The Ohio State University
Su, Chunlei
United States, Knoxville
The University of Tennessee, Knoxville
Dubey, Jitender P.
United States, Beltsville
Usda Ars Beltsville Agricultural Research Center
Statistics
Citations: 35
Authors: 15
Affiliations: 4
Identifiers
Doi:
10.1645/12-25.1
ISSN:
00223395
e-ISSN:
19372345
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Ethiopia