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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Purification and properties of an endoglucanase of Aspergillus terreus DSM 826
Journal of Basic Microbiology, Volume 49, No. 5, Year 2009
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Description
Endoglucanase (EG) from A. terreus DSM 826 grown on sugar cane bagasse as a carbon source was purified using acetone fractionation, then a Sepharose-4B chromatographic column, with purification of about 27-fold and 10.5% recovery. The optimum temperature and pH for activity of the purified EG were found to be 50 °C and pH 4.8, respectively. The purified enzyme can stand heating up to 50 °C for 1 h without apparent loss of activity. However, the enzyme, incubated at 80 °C for 5 min, showed about 56% loss of activity. Optimum EG activity was recorded with a citrate buffer system (pH 4.8; 0.05 M). Co2+ (2.5 × 10-2 M) and Zn2+ (5 × 10-2 M) were found to activate the purified EG of A. terreus DSM 826 by about 83 and 25%, respectively. On the other hand, Hg2+ inhibited the activity of the purified EG by about 50 and 71% at a concentration of 2.5 × 10-2 and 5 × 10-2 M, respectively. Carboxymethyl cellulose was found to be the best substrate for the purified EG, with Vmax values of 4.35 μmol min-1 mg-1 protein. © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Authors & Co-Authors
Elshafei, Ali M.
Egypt, Giza
National Research Centre
Hassan, Mohamed M.
Egypt, Giza
National Research Centre
Haroun, Bakry M.
Egypt, Cairo
Faculty of Science
Abdel-Fatah, Osama Mohamed
Egypt, Giza
National Research Centre
Atta, Houssam Mohamed
Egypt, Cairo
Faculty of Science
Othman, Abdelmageed M.
Egypt, Giza
National Research Centre
Statistics
Citations: 33
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1002/jobm.200800227
ISSN:
0233111X
e-ISSN:
15214028
Research Areas
Environmental