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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
The genetic map of finger millet, Eleusine coracana
Theoretical and Applied Genetics, Volume 114, No. 2, Year 2007
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Description
Restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), expressed-sequenced tag (EST), and simple sequence repeat (SSR) markers were used to generate a genetic map of the tetraploid finger millet (Eleusine coracana subsp. coracana) genome (2n = 4x = 36). Because levels of variation in finger millet are low, the map was generated in an inter-subspecific F2 population from a cross between E. coracana subsp. coracana cv. Okhale-1 and its wild progenitor E. coracana subsp. africana acc. MD-20. Duplicated loci were used to identify homoeologous groups. Assignment of linkage groups to the A and B genome was done by comparing the hybridization patterns of probes in Okhale-1, MD-20, and Eleusine indica acc. MD-36. E. indica is the A genome donor to E. coracana. The maps span 721 cM on the A genome and 787 cM on the B genome and cover all 18 finger millet chromosomes, at least partially. To facilitate the use of marker-assisted selection in finger millet, a first set of 82 SSR markers was developed. The SSRs were identified in small-insert genomic libraries generated using methylation-sensitive restriction enzymes. Thirty-one of the SSRs were mapped. Application of the maps and markers in hybridization-based breeding programs will expedite the improvement of finger millet. © 2006 Springer-Verlag.
Authors & Co-Authors
Dida, Mathews M.
United Kingdom, Norwich
John Innes Centre
United States, Athens
University of Georgia
Kenya, Maseno
Maseno University
Srinivasachary,
United Kingdom, Norwich
John Innes Centre
United States, Athens
University of Georgia
Ramakrishnan, Sujatha
United States, West Lafayette
College of Science
Bennetzen, Jeffrey L.
United States, West Lafayette
College of Science
United States, Athens
University of Georgia
Gále, Michael D.G.
United Kingdom, Norwich
John Innes Centre
Devos, Katrien Martha D.
United Kingdom, Norwich
John Innes Centre
United States, Athens
University of Georgia
Statistics
Citations: 175
Authors: 6
Affiliations: 4
Identifiers
Doi:
10.1007/s00122-006-0435-7
ISSN:
00405752
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study