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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Anti-peptide antibodies differentiate between plasmodial lactate dehydrogenases
Peptides, Volume 31, No. 4, Year 2010
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Description
Malaria lactate dehydrogenase, a glycolytic enzyme, is a malaria diagnostic target in lateral flow immunochromatographic rapid diagnostic tests. Recombinant Plasmodium yoelii LDH was cloned into the pET-28a vector, expressed and the expressed protein purified from a Ni-NTA affinity matrix. A pan-malarial LDH antibody directed against a common malaria LDH peptide (APGKSDKEWNRDDLL) and two anti-peptide antibodies, each targeting a unique Plasmodium falciparum (LISDAELEAIFDC) and Plasmodium vivax (KITDEEVEGIFDC) LDH peptide were raised in chickens. The antibodies were affinity purified with the appropriate peptide affinity matrix. The affinity purified anti-peptide antibodies detected recombinant P. falciparum, P. vivax and P. yoelii LDH and native P. falciparum and P. yoelii LDH in western blots and immunofluorescence studies. The pan-malarial antibody detected LDH from the three malaria species in western blots. The species-specific anti-peptide antibodies differentiated between P. falciparum and P. vivax LDH. Affinity purified chicken antibodies against recombinant PfLDH, PvLDH and PyLDH proteins each detected the parent and orthologous proteins with similar titers in an ELISA. The study supports an anti-peptide antibody approach to the development of diagnostic reagents. © 2010 Elsevier Inc. All rights reserved.
Authors & Co-Authors
Hurdayal, Ramona
South Africa, Durban
University of Kwazulu-natal
Achilonu, Ikechukwu Anthony
South Africa, Durban
University of Kwazulu-natal
Choveaux, David
South Africa, Durban
University of Kwazulu-natal
Coetzer, Theresa HT
South Africa, Durban
University of Kwazulu-natal
Goldring, J. P.Dean
South Africa, Durban
University of Kwazulu-natal
Statistics
Citations: 36
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1016/j.peptides.2010.01.002
ISSN:
01969781
Research Areas
Infectious Diseases