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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Contributions of two nuclear localization signals of influenza A virus nucleoprotein to viral replication
Journal of Virology, Volume 81, No. 1, Year 2007
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Description
The RNA genome of influenza A virus, which forms viral ribonucleoprotein complexes (vRNPs) with viral polymerase subunit proteins (PA, PB1, and PB2) and nucleoprotein (NP), is transcribed and replicated in the nucleas. NP, the major component of vRNPs, has at least two amino acid sequences that serve as nuclear localization signals (NLSs): an unconventional NLS (residues 3 to 13; NLS1) and a bipartite NLS (residues 198 to 216; NLS2). Although both NLSs are known to play role in nuclear transport, their relative contributions to viral replication are poorly understood. We therefore investigated their contributions to NP subcellular/subnuclear localization, viral RNA (vRNA) transcription, and viral replication. Abolishing the unconventional NLS caused NP to localize predominantly to the cytoplasm and affected its activity in vRNA transcription. However, we were able to create a virus whose NP contained amino acid substitutions in NLS1 known to abolish its nuclear localization function, although this virus was highly attenuated. These results indicate that while the unconventional NLS is not essential for viral replication, it is necessary for efficient viral mRNA synthesis. On the other hand, the bipartite NLS, whose contribution to the nuclear transport of NP is limited, was essential for vRNA transcription and NP's nucleolar accumulation. A virus with nonfunctional NLS2 could not be generated. Thus, the bipartite NLS, but not the unconventional NLS, of NP is essential for influenza A virus replication. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Ozawa, Makoto
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Yamada, Shinya
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Yamayoshi, Seiya
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Takada, Ayato
Japan, Sapporo
Hokkaido University
Goto, Hideo
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Horimoto, Taisuke
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
Kawaoka, Yoshihiro K.
Japan, Tokyo
The University of Tokyo
Japan, Kawaguchi
Japan Science and Technology Agency
United States, Madison
University of Wisconsin-madison
Statistics
Citations: 227
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1128/JVI.01434-06
ISSN:
0022538X
Research Areas
Cancer