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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Dissemination of multidrug resistant Acinetobacter baumannii in various hospitals of Antananarivo Madagascar
Annals of Clinical Microbiology and Antimicrobials, Volume 9, Article 17, Year 2010
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Description
This study reports the dissemination of multidrug-resistant (MDR) OXA-23-producing Acinetobacter baumannii clones in hospitals in Antananarivo, Madagascar. A total of 53 carbapenem-resistant A. baumannii isolates were obtained from September 2006 to March 2009 in five hospitals. These resistant strains represent 44% of all A. baumannii isolates. The double disk synergy test was performed to screen for production of metallo-beta-lactamases. Polymerase chain reaction (PCR) and DNA sequencing were performed for the detection of bla (AmpC), bla (OXA-51),bla (OXA-23), bla (OXA-24), bla (IMP), bla (VIM). The presence of the insertion sequence ISAba1 relative to bla OXA-23 and bla OXA-51 was assessed by PCR. Isolates were typed by Rep-PCR. All the isolates were MDR and produced the OXA-23 carbapenemase, which was confirmed by sequencing. PCR analysis for AmpC and OXA-51 gave positive results for all strains studied. No isolates produced metallo-beta-lactamases. In all isolates ISAba1 laid upstream of bla OXA-23. The A. baumannii isolates were separated into two genotypes; genotype A had a higher prevalence (41 strains) than genotype B (12 strains). Genotype A was present in four hospitals, whilst genotype B had spread in two hospitals. The high frequency of MDR OXA-23-producing A. baumannii in various hospitals in Antananarivo is curious since carbapenems are not available in Madagascar, but it emphasises the need for infection control procedures and strict adherence to them to prevent the spread of these resistant organisms in Antananarivo and also the need to control the use of carbapenems in the future.© 2010 Andriamanantena et al; licensee BioMed Central Ltd.
Authors & Co-Authors
Andriamanantena, Tahiry S.
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Ratsima, Elisoa Hariniaina
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Rakotonirina, Hanitra Clara
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Randrianirina, Frédérique
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Ramparany, Lovasoa
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Carod, Jean François
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Richard, Vincent
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Talarmin, Antoine
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Guadeloupe, Pointe-a-pitre
Institut Pasteur de Guadeloupe
Statistics
Citations: 72
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.1186/1476-0711-9-17
e-ISSN:
14760711
Research Areas
Genetics And Genomics
Study Design
Cross Sectional Study
Study Locations
Madagascar