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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
The isolation of high quality RNA from the fruit of avocado (Persea americana Mill.)
South African Journal of Botany, Volume 78, Year 2012
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Description
The fruit of avocado is high in structural carbohydrates and proteins and also contains polyphenols which interfere with the isolation of high quantity and quality of RNA. The aim of this study was to optimize RNA isolation from avocado fruit for subsequent complementary DNA (cDNA) transcription. We describe an optimized cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol that allows effective extraction of high-quality total RNA from the skin and flesh of the fruit without the use of phenol. Total RNA obtained from this procedure is of high quality and is undegraded, as assessed by spectrophotometric readings and electrophoresis, and was successfully used for cDNA synthesis. This is a first report of effective RNA extraction from avocado fruit. © 2011 South African Association of Botanists.
Authors & Co-Authors
Djami-Tchatchou, Arnaud Thierry
South Africa, Johannesburg
University of the Witwatersrand
Straker, Colin John
South Africa, Johannesburg
University of the Witwatersrand
Statistics
Citations: 46
Authors: 2
Affiliations: 1
Identifiers
Doi:
10.1016/j.sajb.2011.04.009
ISSN:
02546299
Research Areas
Genetics And Genomics