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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Epitope analysis of insulin autoantibodies using recombinant Fab
Clinical and Experimental Immunology, Volume 140, No. 3, Year 2005
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Description
Autoantibodies to insulin are often the first autoantibodies detected in young children with type 1 diabetes and can be present before the onset of clinical diabetes. These autoantibodies and their epitopes are, however, not well characterized. We explored the use of monoclonal antibodies and their recombinant Fab as reagents for epitope analysis. In this study we cloned and characterized the recombinant Fab of the insulin-specific monoclonal antibody CG7C7. We found the epitope of this antibody to be located predominantly at the A-chain loop of the insulin molecule. The recombinant Fab was then used to compete for insulin binding against insulin autoantibodies present in sera from patients with type 1 or type 1.5 diabetes. In competition experiments with sera positive for autoantibodies to insulin the recombinant Fab significantly reduced the binding to [125I]-insulin by sera of type 1 (n = 35) and type 1.5 diabetes [latent autoimmune diabetes in adults (LADA)] (n = 14) patients (P < 0.0001). We conclude that competition between insulin-specific monoclonal antibodies or their recombinant Fab and insulin autoantibodies should prove useful in the epitope analysis of autoantibodies to insulin. © 2005 British Society for Immunology.
Authors & Co-Authors
Padoa, Carolyn J.
South Africa, Johannesburg
University of the Witwatersrand
Crowther, Nigel John
South Africa, Johannesburg
University of the Witwatersrand
Thomas, James W.
United States, Nashville
Vanderbilt University
Hall, Tyler R.
United States, Seattle
University of Washington
Bekris, Lynn M.
United States, Seattle
University of Washington
Törn, Carina
Sweden, Lund
Skånes Universitetssjukhus
Landin-Olsson, Mona
Sweden, Lund
Skånes Universitetssjukhus
Örtqvist, Eva
Sweden, Stockholm
Karolinska Institutet
Palmer, Jerry P.
United States, Seattle
University of Washington
Lernmark, Åke
United States, Seattle
University of Washington
Hampe, Christiane Susanne
United States, Seattle
University of Washington
Statistics
Citations: 18
Authors: 11
Affiliations: 5
Identifiers
Doi:
10.1111/j.1365-2249.2005.02802.x
ISSN:
00099104
Research Areas
Maternal And Child Health
Noncommunicable Diseases