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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Changes in fecal microbiota of healthy dogs administered amoxicillin
FEMS Microbiology Ecology, Volume 71, No. 2, Year 2010
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Description
The effect of oral amoxicillin treatment on fecal microbiota of seven healthy adult dogs was determined with a focus on the prevalence of bacterial antibiotic resistance and changes in predominant bacterial populations. After 4-7 days of exposure to amoxicillin, fecal Escherichia coli expressed resistance to multiple antibiotics when compared with the pre-exposure situation. Two weeks postexposure, the susceptibility pattern had returned to pre-exposure levels in most dogs. A shift in bacterial populations was confirmed by molecular fingerprinting of fecal bacterial populations using denaturing gradient gel electrophoresis (PCR-DGGE) of the 16S V3 rRNA gene region. Much of the variation in DGGE profiles could be attributed to dog-specific factors. However, permutation tests indicated that amoxicillin exposure significantly affected the DGGE profiles after controlling for the dog effect (P=0.02), and pre-exposure samples were clearly separated from postexposure samples. Sequence analysis of DGGE bands and real-time PCR quantification indicated that amoxicillin exposure caused a shift in the intestinal ecological balance toward a Gram-negative microbiota including resistant species in the family Enterobacteriaceae. © 2009 Federation of European Microbiological Societies.
Authors & Co-Authors
L'Abée-Lund, Trine Marie
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Sorum, Henning
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Yannarell, Anthony C.
United States, Urbana
University of Illinois Urbana-champaign
Mackie, Roderick Ian
United States, Urbana
University of Illinois Urbana-champaign
Statistics
Citations: 59
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1111/j.1574-6941.2009.00808.x
ISSN:
15746941
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Cross Sectional Study