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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Vaccination of vervet monkeys against cutaneous leishmaniosis using recombinant Leishmania 'major surface glycoprotein' (gp63)
Veterinary Parasitology, Volume 60, No. 3-4, Year 1995
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Description
Vervet monkeys (Cercopithicus aethiops) were shown to give a positive delayed-type hypersensitivity (DHT) reaction to gp63, a major surface glycoprotein of Leishmania parasites, and also produce antibodies to the molecule following a triple vaccination with a total dose of 150 μg of recombinant gp63 mixed with Bacille Calmette Guerin (BCG). However, peripheral blood leucocytes (PBL) from these animals neither proliferated nor produced any interferon-gamma (IFN-γ) following in vitro stimulation with the antigen. Analysis of lymphocyte subsets following vaccination did not reveal any striking phenotypic alteration of cellular sub-populations in PBL. When vaccinated animals were rechallenged, via the needle, with virulent Leishmania major promastigotes containing salivary gland extracts from vector sandflies, only partial protection was achieved. We conclude from these studies that gp63 produced in Escherichia coli is a safe vaccine molecule which gives only partial protection following vaccination in the vervet monkey host. The molecule requires further improvement for vaccine and/or immunodiagnosis application. © 1995.
Authors & Co-Authors
Olobo, Joseph Okao
Kenya, Nairobi
National Museums of Kenya
Anjili, Christopher Omukhango
Kenya, Nairobi
Kenya Medical Research Institute
Gicheru, Michael Muita
Kenya, Nairobi
National Museums of Kenya
Mbati, Peter Amunga
Kenya, Nairobi
Kenya Medical Research Institute
Kariuki, Thomas M.
Kenya, Nairobi
National Museums of Kenya
Githure, John Ichamwenge
Kenya, Nairobi
Kenya Medical Research Institute
Koech, David Kiprotich
Kenya, Nairobi
Kenya Medical Research Institute
McMaster, W. Robert
Canada, Vancouver
The University of British Columbia
Statistics
Citations: 66
Authors: 8
Affiliations: 3
Identifiers
Doi:
10.1016/0304-4017(95)00788-6
ISSN:
03044017