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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Induction of HIV-specific functional immune responses by a multiclade HIV-1 DNA vaccine candidate in healthy Ugandans
Vaccine, Volume 25, No. 45, Year 2007
Notification
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Description
A phase I randomized, double blind, placebo-controlled trial to assess the immunogenicity of a multiclade HIV-1 DNA plasmid vaccine was conducted in 31 HIV-1-negative Ugandans. Following immunization with DNA at 0, 1, and 2 months, the frequency of HIV-specific immune responses was assessed up to 10 months using a standard chromium release assay (CRA), lymphoproliferative assay (LPA), and antibody dependent cell-mediated cytotoxicity assay (ADCC). Seven of 15 (47%) vaccinees demonstrated CTL activity using the CRA to HIV-1 Env B with responses observed 1 month following the second vaccination and as late as 7 months following complete immunization. Additionally, lymphoproliferative reponses were observed in 14/15 vaccinees against p24. No CTL or LPA responses were observed at baseline or in the placebo group. ADCC activity was minimally induced by DNA vaccination. This study demonstrates that immunization with DNA alone induces CTL and lymphoproliferative responses in a population that will participate in a phase IIb study evaluating HIV-1 DNA priming followed by boosting with a replication-defective recombinant adenovirus vector. © 2007 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Eller, Michael A.
Uganda, Kampala
Makerere University
Eller, Leigh Anne
Uganda, Kampala
Makerere University
Opollo, Marc Sam
Uganda, Kampala
Makerere University
Ouma, Benson J.
Uganda, Kampala
Makerere University
Oballah, Peter O.
Uganda, Kampala
Makerere University
Galley, Lynee M.
United States, Rockville
Hjf
Karnasuta, Chitraporn
Thailand, Bangkok
Armed Forces Research Institute of Medical Sciences, Thailand
Kim, Silvia Ratto
Thailand, Bangkok
Armed Forces Research Institute of Medical Sciences, Thailand
Robb, Merlin Lee
United States, Rockville
Hjf
Michael, Nelson L.
United States, Silver Spring
Walter Reed Army Institute of Research
Kibuuka, Hannah N.
Uganda, Kampala
Makerere University
Wabwire-Mangen, Fred
Uganda, Kampala
Makerere University
Graham, Barney S.
United States, Bethesda
National Institutes of Health Nih
Birx, Deborah L.
United States, Atlanta
Centers for Disease Control and Prevention
de Souza, Mark S.
Thailand, Bangkok
Armed Forces Research Institute of Medical Sciences, Thailand
Cox, Josephine H.
United States, Silver Spring
Walter Reed Army Institute of Research
Statistics
Citations: 25
Authors: 16
Affiliations: 6
Identifiers
Doi:
10.1016/j.vaccine.2007.08.056
ISSN:
0264410X
Research Areas
Disability
Genetics And Genomics
Infectious Diseases
Maternal And Child Health
Study Design
Cross Sectional Study