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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Pilot studies for development of an HIV subtype panel for surveillance of global diversity
AIDS Research and Human Retroviruses, Volume 28, No. 6, Year 2012
Notification
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Description
The continued global spread and evolution of HIV diversity pose significant challenges to diagnostics and vaccine strategies. NIAID partnered with the FDA, WRAIR, academia, and industry to form a Viral Panel Working Group to design and prepare a panel of well-characterized current and diverse HIV isolates. Plasma samples that had screened positive for HIV infection and had evidence of recently acquired infection were donated by blood centers in North and South America, Europe, and Africa. A total of 80 plasma samples were tested by quantitative nucleic acid tests, p24 antigen, EIA, and Western blot to assign a Fiebig stage indicative of approximate time from initial infection. Evaluation of viral load using FDA-cleared assays showed excellent concordance when subtype B virus was tested, but lower correlations for subtype C. Plasma samples were cocultivated with phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMCs) from normal donors to generate 30 viral isolates (50-80% success rate for samples with viral load >10,000 copies/ml), which were then expanded to 10 7-10 9 virus copies per ml. Analysis of env sequences showed that sequences derived from cultured PBMCs were not distinguishable from those obtained from the original plasma. The pilot collection includes 30 isolates representing subtypes B, C, B/F, CRF04-cpx, and CRF02-AG. These studies will serve as a basis for the development of a comprehensive panel of highly characterized viral isolates that reflects the current dynamic and complex HIV epidemic, and will be made available through the External Quality Assurance Program Oversight Laboratory (EQAPOL). © Copyright 2012, Mary Ann Liebert, Inc.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3358106/bin/Supp_Data.pdf
Authors & Co-Authors
Manak, Mark M.
United States, Milford
Seracare Life Sciences
United States, Rockville
Hjf
Sina, Silvana T.
United States, Rockville
Hjf
Anekella, Bharathi
United States, Milford
Seracare Life Sciences
Hewlett, Indira K.
United States, Bethesda
Fda Center for Biologics Evaluation and Research
Sanders-Buell, Eric E.
United States, Rockville
Hjf
Ragupathy, Viswanath
United States, Bethesda
Fda Center for Biologics Evaluation and Research
Kim, Jerome Hahn
United States, Silver Spring
Walter Reed Army Institute of Research
Vermeulen, Marion
South Africa, Weltevreden Park
South African National Blood Service
Stramer, Susan L.
United States, Washington, D.c.
American Red Cross
Sabino, Ester Cerdeira
Brazil, Sao Paulo
Universidade de São Paulo
Grabarczyk, Piotr
Poland, Warsaw
Institute of Haematology and Transfusion Medicine, Warsaw
Michael, Nelson L.
United States, Silver Spring
Walter Reed Army Institute of Research
Peel, Sheila A.
United States, Silver Spring
Walter Reed Army Institute of Research
Garrett, Patricia E.
United States, Milford
Seracare Life Sciences
Tovanabutra, Sodsai
United States, Rockville
Hjf
Busch, Michael P.
United States, San Francisco
Vitalant Research Institute
Schito, Marco L.
United States, Bethesda
National Institutes of Health Nih
Statistics
Citations: 19
Authors: 17
Affiliations: 10
Identifiers
Doi:
10.1089/aid.2011.0271
ISSN:
08892229
e-ISSN:
19318405
Research Areas
Infectious Diseases
Study Approach
Quantitative