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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Multiple ciguatoxins present in Indian Ocean reef fish
Toxicon, Volume 40, No. 9, Year 2002
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Description
Optimised gradient reversed-phase high-performance liquid chromatography electrospray ionisation mass spectrometry (LC/MS) methods, in combination with a [3H]-brevetoxin binding assay (RLB), revealed multiple ciguatoxins in a partially purified extract of a highly toxic Lutjanus sebae (red emperor) from the Indian Ocean. Two major ciguatoxins of 1140.6Da (I-CTX-1 and -2) and two minor ciguatoxins of 1156.6Da (I-CTX-3 and -4) were identified. Accurate mass analysis revealed that I-CTX-1 and -2 and Caribbean C-CTX-1 had indistinguishable masses (1140.6316Da, at 0.44ppm resolution). Toxicity estimated from LC/MS/RLB responses indicated that I-CTX-1 and -2 were both ∼60% the potency of Pacific ciguatoxin-1 (P-CTX-1). In contrast to ciguatoxins of the Pacific where the more oxidised ciguatoxins are more potent, I-CTX-3 and -4 were ∼20% of P-CTX-1 potency. Interconversion in dilute acid or on storage, typical of spiroketal and hemiketal functionality found in P-CTXs and C-CTXs, respectively, was not observed to occur between I-CTX-1 and -2. The ratio of CTX-1 and -2 varied depending on the fish extract being analysed. These results suggest that I-CTX-1 and -2 may arise from separate dinoflagellate precursors that may be oxidatively biotransformed to I-CTX-3 and -4 in fish. © 2002 Published by Elsevier Science Ltd.
Authors & Co-Authors
Hamilton, Brett
Australia, Brisbane
The University of Queensland
Australia, Woolloongabba
Queensland Alliance for Environmental Health Sciences
Hurbungs, Mira
Mauritius, Albion
Ministry of Fisheries
Jones, Alun
Australia, Brisbane
The University of Queensland
Lewis, Richard James
Australia, Brisbane
The University of Queensland
Statistics
Citations: 93
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1016/S0041-0101(02)00146-0
ISSN:
00410101
Research Areas
Environmental