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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients
Journal of Immunology, Volume 188, No. 3, Year 2012
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Description
The vitamin D receptor (VDR) gene has been involved in the modulation of susceptibility to inflammatory and autoimmune conditions, and could play a role in the pathogenesis of type 2 diabetes mellitus (T2DM). Susceptibility to T2DM was recently also suggested to associate with HLA alleles. We evaluated possible correlations between VDR polymorphisms, HLA alleles, and risk for development of T2DM by analyzing 627 individuals (368 T2DM patients and 259 healthy control subjects) part of a well-characterized cohort followed in Riyadh, Kingdom of Saudi Arabia. Genomic DNA was genotyped for the VDR gene single nucleotide polymorphisms of Fok-1, Taq-1, ApaI, and Bsm-I. Analyses were run by allelic discrimination real-time PCR. HLA genotyping was performed as well by PCR using sequence-specific primers, whereas cytokine production was evaluated by FACS. Results showed T2DM to be significantly associated with the VDR Taq1 (rs731236-AG) and Bsm-I (rs1544410-CT) genotypes, and the VDR rs1544410-T allele. Cosegregations resulting in significant increases of T2DM odds ratio were detected between Taq1 and Bsm-I VDR polymorphisms and HLA DRB1*04. Notably, the VDR polymorphisms observed to be more frequent in T2DM patients correlated with increased VDR expression and IL-12 production, as well as with metabolic parameters of susceptibility to T2DM, including serum cholesterol and high-density lipoprotein levels. VDR polymorphisms are present in T2DM, and correlate with HLA DRB1*04 and with immunologic and metabolic parameters; results from this study add T2DM to the list of diseases that are likely modulated by an HLA/VDR interaction. Copyright © 2012 by The American Association of Immunologists, Inc.
Authors & Co-Authors
Al-Daghri, N. Mohammad
Saudi Arabia, Riyadh
College of Sciences
Saudi Arabia, Riyadh
King Saud University
Al-Attas, Omar Salem
Saudi Arabia, Riyadh
College of Sciences
Saudi Arabia, Riyadh
King Saud University
Alokail, Majed Saleh
Saudi Arabia, Riyadh
College of Sciences
Saudi Arabia, Riyadh
King Saud University
Alkharfy, Khalid Mohammad
Saudi Arabia, Riyadh
College of Sciences
Saudi Arabia, Riyadh
King Saud University
Saudi Arabia, Riyadh
College of Pharmacy
Draz, Hossam M.
Saudi Arabia, Riyadh
College of Sciences
Agliardi, Cristina
Italy, Milan
Fondazione Don Carlo Gnocchi
Mohammed, Abdul Khader
Saudi Arabia, Riyadh
College of Sciences
Guerini, Franca Rosa
Italy, Milan
Fondazione Don Carlo Gnocchi
Clerici, Mario S.
Saudi Arabia, Riyadh
College of Sciences
Italy, Milan
Fondazione Don Carlo Gnocchi
Italy, Milan
Università Degli Studi Di Milano
Statistics
Citations: 52
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.4049/jimmunol.1101954
ISSN:
00221767
e-ISSN:
15506606
Research Areas
Genetics And Genomics
Noncommunicable Diseases
Study Design
Cohort Study
Case-Control Study