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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
Apoptosis induction by silica nanoparticles mediated through reactive oxygen species in human liver cell line HepG2
Toxicology and Applied Pharmacology, Volume 259, No. 2, Year 2012
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Description
Silica nanoparticles are increasingly utilized in various applications including agriculture and medicine. In vivo studies have shown that liver is one of the primary target organ of silica nanoparticles. However, possible mechanisms of hepatotoxicity caused by silica nanoparticles still remain unclear. In this study, we explored the reactive oxygen species (ROS) mediated apoptosis induced by well-characterized 14. nm silica nanoparticles in human liver cell line HepG2. Silica nanoparticles (25-200μg/ml) induced a dose-dependent cytotoxicity in HepG2 cells. Silica nanoparticles were also found to induce oxidative stress in dose-dependent manner indicated by induction of ROS and lipid peroxidation and depletion of glutathione (GSH). Quantitative real-time PCR and immunoblotting results showed that both the mRNA and protein expressions of cell cycle checkpoint gene p53 and apoptotic genes (bax and caspase-3) were up-regulated while the anti-apoptotic gene bcl-2 was down-regulated in silica nanoparticles treated cells. Moreover, co-treatment of ROS scavenger vitamin C significantly attenuated the modulation of apoptotic markers along with the preservation of cell viability caused by silica nanoparticles. Our data demonstrated that silica nanoparticles induced apoptosis in human liver cells, which is ROS mediated and regulated through p53, bax/bcl-2 and caspase pathways. This study suggests that toxicity mechanisms of silica nanoparticles should be further investigated at in vivo level. © 2012 Elsevier Inc.
Authors & Co-Authors
Ahmad, Javed
Saudi Arabia, Riyadh
College of Sciences
Ahamed, Maqusood Basheer
Saudi Arabia, Riyadh
King Saud University
Akhtar, Mohd Javed
India, Lucknow
Indian Institute of Toxicology Research
Alrokayan, Salman A.H.
Saudi Arabia, Riyadh
King Saud University
A.Siddiqui, Maqsood Ahmed
Saudi Arabia, Riyadh
College of Sciences
Musarrat, Javed
Saudi Arabia, Riyadh
College of Sciences
Al-Khedhairy, Abdulaziz Ali A.
Saudi Arabia, Riyadh
College of Sciences
Statistics
Citations: 196
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/j.taap.2011.12.020
ISSN:
0041008X
e-ISSN:
10960333
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Study Approach
Quantitative