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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Long-term maintenance of polysaccharide-specific antibodies by IgM-secreting cells
Journal of Immunology, Volume 188, No. 1, Year 2012
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Description
Many bacteria-associated polysaccharides induce long-lived Ab responses that protect against pathogenic microorganisms. The maintenance of polysaccharide-specific Ab titers may be due to long-lived plasma cells or ongoing Ag-driven B cell activation due to polysaccharide persistence. BALB/c and V HJ558.3 transgenic mice respond to α1→3-dextran (DEX) by generating a peak anti-DEX response at 7 d, followed by maintenance of serum Ab levels for up to 150 d. Analysis of the cellular response to DEX identified a population of short-lived, cyclophosphamide-sensitive DEX-specific plasmablasts in the spleen, and a quiescent, cyclophosphamide-resistant DEX-specific Ab-secreting population in the bone marrow. BrdU pulse-chase experiments demonstrated the longevity of the DEX-specific Ab-secreting population in the bone marrow. Splenic DEX-specific plasmablasts were located in the red pulp with persisting DEX-associated CD11c + dendritic cells 90 d after immunization, whereas DEX was not detected in the bone marrow after 28 d. Selective depletion of short-lived DEX-specific plasmablasts and memory B1b B cells using cyclophosphamide and anti-CD20 treatment had a minimal impact on the maintenance of serum anti-DEX Abs. Collectively, these findings demonstrate that the maintenance of serum polysaccharide-specific Abs is the result of continuous Ag-driven formation of short-lived plasmablasts in the spleen and a quiescent population of Absecreting cells maintained in the bone marrow for a long duration. Copyright © 2011 by The American Association of Immunologists, Inc.
Authors & Co-Authors
Foote, Jeremy B.
United States, Birmingham
The University of Alabama at Birmingham
Mahmoud, Tamer I.
United States, Birmingham
The University of Alabama at Birmingham
Egypt, Cairo
Faculty of Pharmacy
Vale, André Macedo
United States, Birmingham
The University of Alabama at Birmingham
Kearney, John F.
United States, Birmingham
The University of Alabama at Birmingham
Statistics
Citations: 70
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.4049/jimmunol.1100783
ISSN:
00221767
e-ISSN:
15506606
Research Areas
Maternal And Child Health
Study Design
Cross Sectional Study