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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Signal transducer and activator of transcription 3 is involved in the cardioprotective signalling pathway activated by insulin therapy at reperfusion
Basic Research in Cardiology, Volume 103, No. 5, Year 2008
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Description
Objective: To evaluate the significance of the JAK-STAT pathway in insulin-induced cardioprotection from reperfusion injury. Methods: In isolated perfused rat hearts subjected to insulin therapy (0.3 mU/ml) ± AG490 (5 μM, JAK-STAT inhibitor), the phosphorylation state of STAT3 and Akt was determined after 15 min of reperfusion. Infarct size was measured after 120 min of reperfusion. Isolated cardiac myocytes from wild type (WT) and cardiac specific STAT3 deficient mice were treated with insulin at reoxygenation following simulated ischemia (SI, 26 h). Cell viability was measured after 120 min of reoxygenation following SI, whereas phosphorylation state of Akt was measured after 15 min of reoxygenation following SI. Results: Insulin given at reperfusion led to phosphorylation of STAT3 and Akt both of which were inhibited by AG490. AG490 also blocked the insulin-dependent decrease in infarct size, supporting a role for JAK-STAT in cardioprotection. In addition, insulin protection from SI was blocked in myocytes from the STAT3 deficient mice, or in WT mice treated with AG490. Furthermore, insulin failed to phosphorylate Akt in the STAT3 deficient cardiomyocytes. Conclusion: Insulin-induced cardioprotection at reperfusion occurs through activation of STAT3. Inhibiting STAT3 by AG490, or STAT3 depletion in cardiac myocytes affects activation of Akt, suggesting close interaction between STAT3 and Akt in the cardioprotective signalling pathway activated by insulin treatment at reperfusion. © Springer 2008.
Authors & Co-Authors
Fuglesteg, Britt N.
Norway, Tromso
Uit Norges Arktiske Universitet
Suleman, Naushaad
South Africa, Cape Town
University of Cape Town
Tiron, Crina
Norway, Bergen
Det Medisinske Fakultet
Kanhema, Tambuzai
Norway, Bergen
Det Medisinske Fakultet
Lacerda, Lydia
South Africa, Cape Town
University of Cape Town
Andreasen, Thomas V.
Norway, Tromso
Uit Norges Arktiske Universitet
Sack, Michael N.
United States, Bethesda
National Heart, Lung, and Blood Institute Nhlbi
Jonassen, Anne Kristine
Norway, Bergen
Det Medisinske Fakultet
Mjös, Ole Danbolt
Norway, Tromso
Uit Norges Arktiske Universitet
Opie, LionelH H.
South Africa, Cape Town
University of Cape Town
Lecour, Sandrine C.
South Africa, Cape Town
University of Cape Town
Statistics
Citations: 101
Authors: 11
Affiliations: 4
Identifiers
Doi:
10.1007/s00395-008-0728-x
ISSN:
03008428
Research Areas
Noncommunicable Diseases
Violence And Injury