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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype
Cell Cycle, Volume 8, No. 7, Year 2009
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Description
The potential of mesenchymal stem cells (MSC) to differentiate into neural lineages has raised the possibility of autologous cell transplantation as a therapy for neurodegenerative diseases. We have identified a population of circulating human fetal mesenchymal stem cells (hfMSC) that are highly proliferative and can readily differentiate into mesodermal lineages such as bone, cartilage, fat and muscle. Here, we demonstrate for the first time that primary hfMSC can differentiate into cells with an oligodendrocyte phenotype both in vitro and in vivo. By exposing hfMSC to neuronal conditioned medium or by introducing the pro-oligodendrocyte gene, Olig-2, hfMSC adopted an oligodendrocyte-like morphology, expressed oligodendrocyte markers and appeared to mature appropriately in culture. Importantly we also demonstrate the differentiation of a clonal population of hfMSC into both mesodermal (bone) and ectodermal (oligodendrocyte) lineages. In the developing murine brain transplanted hfMSC integrated into the parenchyma but oligodendrocyte differentiation of these naïve hfMSC was very low. However, the proportion of cells expressing oligodendrocyte markers increased significantly (from 0.2% to 4%) by pre-exposing the cells to differentiation medium in vitro prior to transplantation. Importantly, the process of in vivo differentiation occurred without cell fusion. These findings suggest that hfMSC may provide a potential source of oligodendrocytes for study and potential therapy. ©2009 Landes Bioscience.
Authors & Co-Authors
Waddington, Simon N.
United Kingdom, London
Imperial College London
Chan, Jerry Kok Yen
United Kingdom, London
Imperial College London
Al-Allaf, Faisal Ahmad
United Kingdom, London
Imperial College London
Themis, Michael
United Kingdom, London
Imperial College London
Edwards, Anthony David
United Kingdom, London
Mrc Clinical Sciences Centre
Fisk, N. M.
United Kingdom, London
Imperial College London
Mehmet, Huseyin
United Kingdom, London
Imperial College London
United States, Rahway
Merck & Co., Inc.
Statistics
Citations: 61
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.4161/cc.8.7.8121
ISSN:
15384101
Research Areas
Cancer
Genetics And Genomics
Study Design
Cross Sectional Study