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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
chemical engineering
Production of laccase by a newly isolated strain of Trametes modesta
Bioresource Technology, Volume 84, No. 3, Year 2002
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Description
The effects of the carbon and nitrogen sources, initial pH and incubation temperature on laccase production by Trametes modesta were evaluated using the one-factor-at-a-time method. The final optimisation was done using a central composite design resulting in a four-fold increase of the laccase activity to 178 nkat ml-1. Response-surface analysis showed that 7.34 g l-1 wheat bran, 0.87 g l-1 glucose, 2.9 g l-1 yeast extract, 0.25 g l-1 ammonium chloride, an initial pH of 6.95 and an incubation temperature of 30.26°C were the optimal conditions for laccase production. Laccase produced by T. modesta was fully active at pH 4 and at 50°C. The laccase was very stable at pH 4.5 and at 40°C but half-lives decreased to 120 and 125 min at higher temperature (60°C) and lower pH (pH 3). © 2002 Elsevier Science Ltd. All rights reserved.
Authors & Co-Authors
Nyanhongo, Gibson Stephen
Austria, Graz
Technische Universitat Graz
Gomes, J.
Austria, Graz
Technische Universitat Graz
Guebitz, Georg M.
Austria, Graz
Technische Universitat Graz
Zvauya, Remigio
Zimbabwe, Harare
University of Zimbabwe
Read, John S.
Zimbabwe, Bulawayo
National University of Science and Technology Bulawayo
Steiner, Walter
Austria, Graz
Technische Universitat Graz
Statistics
Citations: 147
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/S0960-8524(02)00044-5
ISSN:
09608524
Research Areas
Environmental