Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Diarrheagenic Escherichia coli detected by 16-plex PCR in raw meat and beef intestines sold at local markets in Ouagadougou, Burkina Faso
International Journal of Food Microbiology, Volume 153, No. 1-2, Year 2012
Notification
URL copied to clipboard!
Description
The study investigated the prevalence of five major Escherichia coli pathogroups in raw meats and beef intestines sold at the local markets in Ouagadougou, Burkina Faso. One hundred and twenty samples (36 beef, 36 beef intestine, 24 mutton and 24 chicken samples) were purchased from four markets between October 2008 and February 2009. Fifteen virulence genes specific for Shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and enteroaggregative E. coli (EAEC) were examined using 16-plex PCR for mixed bacterial cultures derived from the samples. One or more diarrheagenic E. coli pathogroup was detected in 51 (43%) of all the 120 samples: in 16 (44%) beef, 19 (53%) beef intestine, 9 (38%) mutton and in 7 (29%) chicken samples. Thirty three (28%) samples were positive for stx 1 and/or stx 2 indicating presence of STEC. EPEC virulence markers (eae, escV and/or ent and/or bfp and/or EHEC-hlyA) were detected in 14 (12%) stx-negative samples. ETEC virulence markers (elt and/or estIb and/or estIa) were detected in 10 (8%) samples and EAEC virulence markers (pic or aggR) in 5 (4%) samples. No EIEC was detected. The results show that in Burkina Faso the microbiological quality of retail meat is alarmingly poor due to the common occurrence of diarrheagenic E. coli bacteria. © 2011 Elsevier B.V.
Authors & Co-Authors
Kagambèga, Assèta
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Burkina Faso, Ouagadougou
University of Ouagadougou
Martikainen, Outi
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Lienemann, Taru
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Siitonen, Anja
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Traoré, Alfred Sababénédjo
Burkina Faso, Ouagadougou
University of Ouagadougou
Barro, Nicolas
Burkina Faso, Ouagadougou
University of Ouagadougou
Haukka, Kaisa E.
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Statistics
Citations: 51
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.ijfoodmicro.2011.10.032
ISSN:
01681605
Study Design
Cross Sectional Study
Study Locations
Burkina Faso