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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Isolation of viral igy antibodies from yolks of immunized hens
Immunological Investigations, Volume 9, No. 5, Year 1980
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Description
Antibodies were isolated from the yolks of hens that were immunized with a variety of plant viruses by the use of polyethylene glycol (PEG). A concentration of 3.5% of the polymer caused the lipids and vitellin to separate, and the IgY was then precipitated with 12% PEG. The titre of the isolated antibody appears to remain at a high level after cessation of the course of immunization. Antibodies derived from the yolks of hens appear to have titres similar to those found in serum of rabbits immunized simultaneously. The observation made by several authors that a high salt concentration enhances fowl serum antibody precipitin titres could not be corroborated with 'yolk' antibodies directed against several plant viruses. © 1980 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Authors & Co-Authors
Polson, Alfred
South Africa, Cape Town
University of Cape Town
von Wechmar, M. Barbara
South Africa, Cape Town
University of Cape Town
van Regenmortel, Marc H.V.
France, Strasbourg
Institut de Biologie Moléculaire et Cellulaire
Statistics
Citations: 448
Authors: 3
Affiliations: 2
Identifiers
Doi:
10.3109/08820138009066010
ISSN:
08820139
Research Areas
Maternal And Child Health