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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Differences in innate cytokine responses between European and African children
PLoS ONE, Volume 9, No. 4, Article e95241, Year 2014
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Description
Although differences in immunological responses between populations have been found in terms of vaccine efficacy, immune responses to infections and prevalence of chronic inflammatory diseases, the mechanisms responsible for these differences are not well understood. Therefore, innate cytokine responses mediated by various classes of patternrecognition receptors including Toll-like receptors (TLR), C-type lectin receptors (CLRs) and nucleotide-binding oligomerisation domain-like receptors (NLRs) were compared between Dutch (European), semi-urban and rural Gabonese (African) children. Whole blood was stimulated for 24 hours and the pro-inflammatory tumor necrosis factor (TNF) and the anti-inflammatory/regulatory interleukin-10 (IL-10) cytokines in culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). Gabonese children had a lower pro-inflammatory response to poly(I:C) (TLR3 ligand), but a higher pro-inflammatory response to FSL-1 (TLR2/6 ligand), Pam3 (TLR2/1 ligand) and LPS (TLR4 ligand) compared to Dutch children. Anti-inflammatory responses to Pam3 were also higher in Gabonese children. Non-TLR ligands did not induce substantial cytokine production on their own. Interaction between various TLR and non-TLR receptors was further assessed, but no differences were found between the three populations. In conclusion, using a field applicable assay, significant differences were observed in cytokine responses between European and African children to TLR ligands, but not to non-TLR ligands.©2014 Labuda et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3990610/bin/pone.0095241.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3990610/bin/pone.0095241.s002.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3990610/bin/pone.0095241.s003.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC3990610/bin/pone.0095241.s004.tif
Authors & Co-Authors
Labuda, Lucja A.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Gabon, Lambarene
Centre de Recherches Médicales de Lambaréné
Germany, Tubingen
Eberhard Karls Universität Tübingen
De Jong, Sanne E.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Gabon, Lambarene
Centre de Recherches Médicales de Lambaréné
Meurs, Lynn
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Amoah, Abena Serwaa
Netherlands, Leiden
Leids Universitair Medisch Centrum
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Mbow, Moustapha
Netherlands, Leiden
Leids Universitair Medisch Centrum
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Senegal, Dakar
Aristide le Dantec Teaching Hospital
Ateba-Ngoa, Ulysse
Netherlands, Leiden
Leids Universitair Medisch Centrum
Gabon, Lambarene
Centre de Recherches Médicales de Lambaréné
Germany, Tubingen
Eberhard Karls Universität Tübingen
Van Der Ham, Alwin J.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Knulst, André Cornelis
Netherlands, Utrecht
University Medical Center Utrecht
Yazdanbakhsh, Maria M.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Adégnika, Ayôla Akim
Netherlands, Leiden
Leids Universitair Medisch Centrum
Gabon, Lambarene
Centre de Recherches Médicales de Lambaréné
Germany, Tubingen
Eberhard Karls Universität Tübingen
Statistics
Citations: 29
Authors: 10
Affiliations: 7
Identifiers
Doi:
10.1371/journal.pone.0095241
e-ISSN:
19326203
Research Areas
Cancer
Maternal And Child Health
Study Design
Cross Sectional Study