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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
A continuously monitored colorimetric method for detection of Mycobacterium tuberculosis complex in sputum
International Journal of Tuberculosis and Lung Disease, Volume 17, No. 12, Year 2013
Notification
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Description
SETTING: Mbeya, Tanzania. OBJECTIVE: To develop a new liquid culture method to detect Mycobacterium tuberculosis complex (MTC) in sputum using 2,3-diphenyl-5-thienyl-(2)-tetrazolium (STC), the nitrate reductase assay (NRA) and p-nitro-benzoic acid (PNB). DESIGN: Ninety-three sputum samples collected from 18 tuberculosis patients were decontaminated with N-acetyl-L-cysteine- sodium hydroxide using MGIT™ 960 and in STC-NRA cultures, both in the presence and in the absence of PNB, an inhibitor of MTC growth. The reduction of STC by colour change indicated mycobacterial growth; NRA was then performed to confirm MTC. RESULTS: STC-NRA culture was positive for acid-fast bacilli in 66/93 (71%) samples, of which 60/93 (64.5%) were identified as MTC-positive and 6/93 (6.5%) as indeterminate mycobacteria. MGIT indicated MTC in 59/93 (63.4%) cultures. Contamination was detected in 12/93 (13%) STC-NRA cultures vs. 29/93 (31.2%) MGIT cultures. The mean time to detection (TTD) of MTC using STC-NRA was 14 days and 7 days using MGIT. CONCLUSION: The STC-NRA method is sensitive for the detection of MTC in sputum. TTD increased with duration of anti-tuberculosis treatment, highlighting the value of this method in monitoring treatment success. The method is simple and inexpensive and, unlike MGIT, does not require technical equipment. The preliminary performance characteristics of the method should be further evaluated in larger studies. © 2013 The Union.
Authors & Co-Authors
Rojas-Ponce, Gabriel
Tanzania
National Institute for Medical Research Tanzania
Rachow, Andrea
Germany, Munich
Klinikum Der Universität München
Germany, Munich
German Center for Infection Research
Guerra Allison, Humberto
Peru, Lima
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt
Mapamba, Daniel Adon
Tanzania
National Institute for Medical Research Tanzania
Joseph, Jeyasekaran
Tanzania
National Institute for Medical Research Tanzania
Mlundi, R.
Tanzania
National Institute for Medical Research Tanzania
Marimoto, S.
Tanzania
National Institute for Medical Research Tanzania
Ntinginya, Nyanda Elias
Tanzania
National Institute for Medical Research Tanzania
Mangu, Chacha D.
Tanzania
National Institute for Medical Research Tanzania
Framhein, A.
Tanzania
National Institute for Medical Research Tanzania
Butler, A.
Tanzania
National Institute for Medical Research Tanzania
Kohlenberg, Anke
Tanzania
National Institute for Medical Research Tanzania
Germany, Munich
Klinikum Der Universität München
Ngatemelela, D.
Tanzania
National Institute for Medical Research Tanzania
Froeschl, Guenter
Germany, Munich
Klinikum Der Universität München
Maboko, Leonard L.
Tanzania
National Institute for Medical Research Tanzania
Höelscher, Michael
Tanzania
National Institute for Medical Research Tanzania
Germany, Munich
Klinikum Der Universität München
Germany, Munich
German Center for Infection Research
Heinrich, Norbert
Germany, Munich
Klinikum Der Universität München
Germany, Munich
German Center for Infection Research
Statistics
Citations: 17
Authors: 17
Affiliations: 4
Identifiers
Doi:
10.5588/ijtld.13.0317
ISSN:
10273719
Study Locations
Tanzania