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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
A generalised transducing bacteriophage for Rhodococcus erythropolis
MGG Molecular & General Genetics, Volume 206, No. 1, Year 1987
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Description
Q4, a bacteriophage isolated from soil, mediated the transduction of a number of unlinked markers in Rhodococcus erythropolis. Highest numbers of transductants were obtained at multiplicities of infection of over 100, transductants only being obtained because of the temperate nature of the phage. Under optimal conditions, transduction to prototrophy of auxotrophic markers was over 50 times the spontaneous reversion rate and transduction of some antibitic resistance markers was over 10 times the spontaneous mutation rate. Segregation of unselected, but linked, markers was observed and the phage was used to order loci in a three factor cross. The virus required magnesium ions. Highest phage titres and greatest transduction frequency were obtained with stationary phase cultures. © 1987 Springer-Verlag.
Authors & Co-Authors
Dabbs, Eric R.
South Africa, Johannesburg
Csir Laboratory for Molecular and Cell Biology
South Africa, Pretoria
Microbiology Research Group Pretoria
Statistics
Citations: 7
Authors: 1
Affiliations: 2
Identifiers
Doi:
10.1007/BF00326545
ISSN:
00268925
e-ISSN:
16174623
Research Areas
Cancer
Genetics And Genomics