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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinella aurita) by-products proteins
Food Chemistry, Volume 118, No. 3, Year 2010
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Description
In order to utilise sardinelle (Sardinella aurita) protein by-products, which is normally discarded as industrial waste in the process of fish manufacturing, heads and viscera proteins were hydrolysed by different proteases to obtain antioxidative peptides. All hydrolysates showed different degrees of hydrolysis and varying degrees of antioxidant activities. Hydrolysate generated with crude enzyme extract from sardine (Sardina pilchardus) displayed high antioxidant activity, and the higher DPPH radical-scavenging activity (87 ± 2.1% at 2 mg/ml) was obtained with a degree of hydrolysis of 6%. This hydrolysate was fractionated by size exclusion chromatography on a Sephadex G-25 into eight major fractions (P1-P8). Fraction P4, which exhibited the highest DPPH scavenging activity, was then fractionated by reversed-phase high performance liquid chromatography (RP-HPLC). Seven antioxidant peptides were isolated. The molecular masses and amino acids sequences of the purified peptides were determined using ESI-MS and ESI-MS/MS, respectively. Their structures were identified as Leu-His-Tyr, Leu-Ala-Arg-Leu, Gly-Gly-Glu, Gly-Ala-His, Gly-Ala-Trp-Ala, Pro-His-Tyr-Leu and Gly-Ala-Leu-Ala-Ala-His. The first peptide displayed the highest DPPH radical-scavenging activity (63 ± 1.57%; at 150 μg/ml) among these peptides. The results of this study suggest that sardinelle by-products protein hydrolysates are good source of natural antioxidants. © 2009 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Bougatef, Ali
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Nedjar-Arroume, Naïma
France, Villeneuve-d'ascq
Institut Charles Viollette
Manni, Laila
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Ravallec, Rozenn
France, Villeneuve-d'ascq
Institut Charles Viollette
Barkia, Ahmed
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Guillochon, Didier
France, Villeneuve-d'ascq
Institut Charles Viollette
Nasri, Moncef
Tunisia, Sfax
Ecole Nationale D'ingénieurs de Sfax
Statistics
Citations: 419
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.foodchem.2009.05.021
ISSN:
03088146